Histochemistry and Cell Biology

, Volume 130, Issue 3, pp 435–445

RNA expression analysis from formalin fixed paraffin embedded tissues

  • Susan M. Farragher
  • Austin Tanney
  • Richard D. Kennedy
  • D. Paul Harkin
Review

DOI: 10.1007/s00418-008-0479-7

Cite this article as:
Farragher, S.M., Tanney, A., Kennedy, R.D. et al. Histochem Cell Biol (2008) 130: 435. doi:10.1007/s00418-008-0479-7

Abstract

Formalin fixation and paraffin embedding (FFPE) is the most commonly used method worldwide for tissue storage. This method preserves the tissue integrity but causes extensive damage to nucleic acids stored within the tissue. As methods for measuring gene expression such as RT-PCR and microarray are adopted into clinical practice there is an increasing necessity to access the wealth of information locked in the Formalin fixation and paraffin embedding archives. This paper reviews the progress in this field and discusses the unique opportunities that exist for the application of these techniques in the development of personalized medicine.

Keywords

FFPE RNA profiling Microarray Gene expression Personalized medicine 

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Susan M. Farragher
    • 1
  • Austin Tanney
    • 1
  • Richard D. Kennedy
    • 1
    • 2
  • D. Paul Harkin
    • 1
    • 3
  1. 1.Almac DiagnosticsCraigavonArmaghUK
  2. 2.Belfast City Hospital TrustBelfastUK
  3. 3.Centre for Cancer Research and Cell BiologyQueen’s University BelfastBelfastUK

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