Histochemistry and Cell Biology

, Volume 130, Issue 4, pp 709–717

Heterotypic tubular connections at the endoplasmic reticulum–Golgi complex interface

  • Guillermo Vivero-Salmerón
  • José Ballesta
  • José A. Martínez-Menárguez
Original Paper

DOI: 10.1007/s00418-008-0471-2

Cite this article as:
Vivero-Salmerón, G., Ballesta, J. & Martínez-Menárguez, J.A. Histochem Cell Biol (2008) 130: 709. doi:10.1007/s00418-008-0471-2


Electron microscopy and cryoimmunocytochemistry were used to characterize tubular connections in the secretory pathway using rat spermatids as model. Our results support the existence of a complex tubular network enriched in the Golgi matrix protein GM130 that transiently joins the cis-Golgi side and the endoplasmic reticulum. These tubules occasionally contain the endoplasmic reticulum resident protein PDI but not COPII complexes or KDEL receptor. At the lateral edges of the stacks tubules were seen to connect cisternae belonging to the same or adjacent stacks. These connections were observed in all cisternae but preferentially on the cis side. Giantin, Gos28 and Rab6 were detected in the tubules; importantly, we reported the presence of cistrans heterotypic connections between cisternae. On the trans-Golgi side, we occasionally observed tubules highly immunoreactive for Rab6 connecting the stack with the forming acrosome. Together, our results support the existence of transient continuities throughout the secretory pathways.


Golgi complexSpermatidTubuleElectron microscopyCryoimmunocytochemistry



cis-Golgi network


Endoplasmic reticulum


Endoplasmic reticulum–Golgi intermediate compartment


Protein disulfide isomerase


trans-Golgi network

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Guillermo Vivero-Salmerón
    • 1
  • José Ballesta
    • 1
  • José A. Martínez-Menárguez
    • 1
  1. 1.Department of Cell Biology and Histology, School of MedicineUniversity of MurciaMurciaSpain