Histochemistry and Cell Biology

, Volume 130, Issue 2, pp 387–397

Expression pattern of serine protease inhibitor kazal type 3 (Spink3) during mouse embryonic development

  • Jun Wang
  • Masaki Ohmuraya
  • Masahiko Hirota
  • Hideo Baba
  • Gang Zhao
  • Motohiro Takeya
  • Kimi Araki
  • Ken-ichi Yamamura
Original Paper

DOI: 10.1007/s00418-008-0425-8

Cite this article as:
Wang, J., Ohmuraya, M., Hirota, M. et al. Histochem Cell Biol (2008) 130: 387. doi:10.1007/s00418-008-0425-8

Abstract

Recent evidence shows that the serine protease inhibitor Kazal type 3 (Spink3) has more diverse functions than expected. To gain insight into its function, we analyzed the spatiotemporal expression profile of Spink3, using in situ hybridization (ISH) and a Spink3+/lacZ knock-in mouse, in which lacZ was inserted into the Spink3 locus. Spink3lacZ expression was first observed in the foregut, midgut, hindgut and the forebrain/midbrain junction region at 9.5 days post coitus (dpc). In the pancreas, Spink3 mRNA was detected at 11.5 dpc, before formation of the typical shape of the exocrine structure of the pancreas. Acinar cell expression was clearly identified by 13.5 dpc. After differentiation of the intestinal tract, Spink3lacZ expression was observed in the large intestine at 11.5 dpc, followed by expression in the small intestine at 13.5 dpc, before appearance of intestinal digestive enzymes. Spink3 mRNA and Spink3lacZ activity were also detected in other tissues, including the mesonephric tubules and the urogenital ridge at 11.5 dpc, the genital swelling at 13.5 dpc, the ductus epididymis at 17.5 dpc, and the seminal vesicle at 8 weeks. These data suggest that Spink3 may play important roles in proliferation and/or differentiation of various cell types during development.

Keyword

Spink3

Abbreviations

Spink3

Serine protease inhibitor Kazal type 1

PSTI

Pancreatic secretory trypsin inhibitor

EGF

Epidermal growth factor

X-gal

4-Chloro-5-bromo-3-indolyl-β-D-galactopyranoside

CCK

Cholecystokinin

ES

Embryonic stem

ISH

In situ hybridization

dpc

Days post coitum

dpp

Days post partum

DIG

Digoxigenin

RE

Right element mutant lox site

TATI

Tumor associated trypsin inhibitor

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Jun Wang
    • 1
  • Masaki Ohmuraya
    • 1
    • 2
  • Masahiko Hirota
    • 2
  • Hideo Baba
    • 2
  • Gang Zhao
    • 1
  • Motohiro Takeya
    • 3
  • Kimi Araki
    • 1
  • Ken-ichi Yamamura
    • 1
  1. 1.Division of Developmental Genetics, Institute of Molecular Embryology and GeneticsKumamoto UniversityKumamotoJapan
  2. 2.Department of Surgery, Faculty of Medical and Pharmaceutical ScienceKumamoto UniversityKumamotoJapan
  3. 3.Division of Cell Pathology, Faculty of Medical and Pharmaceutical ScienceKumamoto UniversityKumamotoJapan