Histochemistry and Cell Biology

, Volume 129, Issue 4, pp 479–487

Partial persistence of exercise-induced myocardial angiogenesis following 4-week detraining in the rat

  • Marina Marini
  • Elisabetta Falcieri
  • Vittoria Margonato
  • Davide Treré
  • Rosa Lapalombella
  • Simona di Tullio
  • Cosetta Marchionni
  • Sabrina Burattini
  • Michele Samaja
  • Fabio Esposito
  • Arsenio Veicsteinas
Original Paper

DOI: 10.1007/s00418-007-0373-8

Cite this article as:
Marini, M., Falcieri, E., Margonato, V. et al. Histochem Cell Biol (2008) 129: 479. doi:10.1007/s00418-007-0373-8
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Abstract

Enhanced angiogenesis, or capillary growth, has a prominent role among the various beneficial effects of exercise training on the myocardium. The aim of the present study is to assess if training-induced increases in capillarity and vascularization persist after 4 weeks of detraining. Adult male rats were trained to run on a treadmill for 10 weeks at ∼60% VO2max, which did not induce cardiac hypertrophy, but increased (P < 0.05) the soleus/body weight ratio, left ventricle capillarity and von Willebrand-positive cell density (n = 6). In another group of animals (n = 6) subjected to training followed by 4-week detraining, the soleus/body weight ratio returned to normal, with only partial reversal of left ventricle capillarity and von Willebrand-positive cell density. Markers of angiogenesis (VEGF, KDR/VEGF-R2 and HIF-1α mRNA, studied by real-time RT-PCR) were upregulated at the end of training, and returned to baseline value after detraining. Electron microscopy highlighted some morphological features in trained hearts (endothelial cell sprouting and bridges and pericyte detachment), suggestive of endothelial cell proliferation and capillary growth that were absent in untrained and detrained hearts. We conclude that the training-induced increase in cardiac capillarity and vascularization are retained for some time upon cessation of the training program even in the absence of angiogenic stimuli.

Keywords

Angiogenesis Exercise training Cardioprotection Rats Electron microscopy 

Abbreviations

VEGF

Vascular endothelial growth factor

VEGF-R2

Vascular endothelial growth factor receptor 2

bFGF

Basic fibroblast growth factor

HIF-1

Hypoxia-inducible factor-1

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Marina Marini
    • 1
    • 2
  • Elisabetta Falcieri
    • 3
    • 4
  • Vittoria Margonato
    • 5
  • Davide Treré
    • 6
  • Rosa Lapalombella
    • 1
  • Simona di Tullio
    • 1
  • Cosetta Marchionni
    • 1
  • Sabrina Burattini
    • 3
  • Michele Samaja
    • 7
  • Fabio Esposito
    • 5
  • Arsenio Veicsteinas
    • 2
    • 5
    • 8
  1. 1.Department of Histology, Embryology and Applied BiologyUniversity of BolognaBolognaItaly
  2. 2.Istituto Interuniversitario di MiologiaChietiItaly
  3. 3.Institute of Morphological SciencesUniversity of UrbinoUrbinoItaly
  4. 4.IGM-CNR, Istituti Ortopedici RizzoliBolognaItaly
  5. 5.Institute of Physical Exercise, Health and Sport ActivitiesUniversity of MilanMilanItaly
  6. 6.Department of Experimental PathologyUniversity of BolognaBolognaItaly
  7. 7.Department of Medicine, Surgery and DentistryUniversity of MilanMilanItaly
  8. 8.Center of Sport Medicine, Don Gnocchi FoundationMilanItaly

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