Alpha smooth muscle actin distribution in cytoplasm and nuclear invaginations of connective tissue fibroblasts
- First Online:
- Cite this article as:
- Storch, K.N., Taatjes, D.J., Bouffard, N.A. et al. Histochem Cell Biol (2007) 127: 523. doi:10.1007/s00418-007-0275-9
- 327 Views
Alpha smooth muscle actin (α-SMA) was recently shown to be present in mouse subcutaneous tissue fibroblasts in the absence of tissue injury. In this study, we used a combination of immunohistochemistry and correlative confocal scanning laser and electron microscopy to investigate the structural organization of α-SMA in relation to the nucleus. Furthermore, we explored colocalization analysis as a method for quantifying the amount of α-SMA in close approximation to the nucleic acid marker, 4′,6-diamidino-2-phenyl-indole, dihydrochloride. Our findings indicate the presence of α-SMA within nuclear invaginations in close proximity to the nuclear membrane, but not in the nucleoplasm. Although the function of these α-SMA-rich nuclear invaginations is at present unknown, the morphology of these structures suggests their possible involvement in cellular and nuclear mechanotransduction as well as nuclear transport.