Histochemistry and Cell Biology

, Volume 127, Issue 2, pp 139–148

Nuclear myosin is ubiquitously expressed and evolutionary conserved in vertebrates

Authors

  • M. Kahle
    • Institute of Experimental Medicine, Department of Cell Ultrastructure and Molecular BiologyAcademy of Sciences of the Czech Republic
    • Institute of Molecular Genetics, Department of Biology of the Cell NucleusAcademy of Sciences of the Czech Republic
  • J. Přidalová
    • Institute of Experimental Medicine, Department of Cell Ultrastructure and Molecular BiologyAcademy of Sciences of the Czech Republic
  • M. Špaček
    • Institute of Experimental Medicine, Department of Cell Ultrastructure and Molecular BiologyAcademy of Sciences of the Czech Republic
    • Department of Histology and Embryology, 3rd Faculty of MedicineCharles University
  • R. Dzijak
    • Institute of Experimental Medicine, Department of Cell Ultrastructure and Molecular BiologyAcademy of Sciences of the Czech Republic
    • Institute of Experimental Medicine, Department of Cell Ultrastructure and Molecular BiologyAcademy of Sciences of the Czech Republic
    • Institute of Molecular Genetics, Department of Biology of the Cell NucleusAcademy of Sciences of the Czech Republic
Original Paper

DOI: 10.1007/s00418-006-0231-0

Cite this article as:
Kahle, M., Přidalová, J., Špaček, M. et al. Histochem Cell Biol (2007) 127: 139. doi:10.1007/s00418-006-0231-0

Abstract

Nuclear myosin I (NMI) is a single-headed member of myosin superfamily localized in the cell nucleus which participates along with nuclear actin in transcription and chromatin remodeling. We demonstrate that NMI is present in cell nuclei of all mouse tissues examined except for cells in terminal stages of spermiogenesis. Quantitative PCR and western blots demonstrate that the expression of NMI in tissues varies with the highest levels in the lungs. The expression of NMI is lower in serum-starved cells and it increases after serum stimulation. The lifespan of NMI is longer than 16 h as determined by cycloheximide translation block. A homologous protein is expressed in human, chicken, Xenopus, and zebrafish as shown by RACE analysis. The analysis of genomic sequences indicates that almost identical homologous NMI genes are expressed in mammals, and similar NMI genes in vertebrates.

Keywords

Nuclear myosin IMyosin 1cTranscriptionChromatinTissue expression

Copyright information

© Springer-Verlag 2006