Histochemistry and Cell Biology

, Volume 126, Issue 1, pp 9–16

Colocalization between caveolin isoforms in the intestinal smooth muscle and interstitial cells of Cajal of the Cav1+/+ and Cav1−/− mouse

Original paper

DOI: 10.1007/s00418-005-0128-3

Cite this article as:
Cho, W.J. & Daniel, E.E. Histochem Cell Biol (2006) 126: 9. doi:10.1007/s00418-005-0128-3

Abstract

Confocal microscopic images were obtained from the immunohistochemical sections of jejeunum to determine the localization/colocalization between caveolin-1, caveolin-2 and caveolin-3 in intestinal smooth muscle cells (SMCs) and interstitial cells of Cajal (ICC) of Cav1+/+ and Cav1−/− mouse. Intestinal regions were segmented [inner circular muscle (icm), outer circular muscle (ocm), myenteric plexus region (mp), and longitudinal muscle (lm)] by LSM 5 and analyzed by ImageJ to show Pearson’s correlation (rp) and overlap coefficient (r) of colocalization. In the intestine of Cav1+/+, caveolin-1 (cav1) was colocalized with caveolin-2 (cav2) and caveolin-3 (cav3). Cav2 also was well colocalized with cav3. In the intestine of Cav1−/−, cav1 and cav2 were absent in all images, but reduced cav3 was expressed in ocm. Caveolae were present in cell types with cav1 in Cav1+/+, and present with cav3 in ocm of Cav1−/−. C-kit occurred in deep muscular plexus (ICC-DMP) and myenteric plexus (ICC-MP), in both Cav1+/+ and Cav1−/−, and colocalized with cav1 and cav2 in the intestine of Cav1+/+. Cav3 was absent/present at low immunoreactivity in ICC-DMP and ICC-MP of the intestines of Cav1+/+ and Cav1−/−. To conclude, cav1 is necessary for the expression of cav2 in SMC and ICC of intestine and facilitates, but is not necessary for the expression of cav3.

Keywords

Caveolin-isoforms Intestinal smooth muscle Interstitial cells of Cajal Segmentation Colocalization 

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  1. 1.Department of Pharmacology, Faculty of Medicine and DentistryUniversity of AlbertaEdmontonCanada

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