Histochemistry and Cell Biology

, Volume 122, Issue 6, pp 521–526

A major site of expression of the ets transcription factor Elf5 is epithelia of exocrine glands

  • Erika J. Lapinskas
  • Jodie Palmer
  • Sharon Ricardo
  • Paul J. Hertzog
  • Annet Hammacher
  • Melanie A. Pritchard
Original Paper

DOI: 10.1007/s00418-004-0713-x

Cite this article as:
Lapinskas, E.J., Palmer, J., Ricardo, S. et al. Histochem Cell Biol (2004) 122: 521. doi:10.1007/s00418-004-0713-x

Abstract

Elf5 belongs to the ets family of transcription factors and was cloned by homology in the DNA binding domain to the related, epithelial-specific ets factor, Elf3. Elf5 mRNA is expressed highly in normal tissue rich in secretory epithelial cells, including mammary gland, lung, kidney, prostate, salivary gland and stomach. The function of Elf5 and the cell types in which it is expressed remain uncharacterised. The presence of Elf5 mRNA in normal tissues, but absence in cancer tissues, may suggest a role for Elf5 in differentiation and development. We have generated a rabbit antiserum directed against a peptide in the Elf5 DNA-binding domain that is conserved between murine and human sequences. The antiserum specifically detects human and murine Elf5 proteins on western blots and shows specific staining on paraffin-embedded sections obtained from tissues including mammary gland, kidney, salivary gland and stomach. Epithelia from the bladder lining, lung and prostate did not stain for the presence of Elf5, though these organs express Elf5 mRNA. We show for the first time that Elf5 is primarily expressed in epithelial cells and is likely to be an epithelial-specific protein. The antiserum should prove useful in further analysis of the expression and function of Elf5.

Keywords

Elf5 Transcription factor Epithelium Antibody 

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Erika J. Lapinskas
    • 1
  • Jodie Palmer
    • 1
    • 3
  • Sharon Ricardo
    • 2
  • Paul J. Hertzog
    • 1
  • Annet Hammacher
    • 1
    • 4
  • Melanie A. Pritchard
    • 1
  1. 1.Centre for Functional Genomics and Human Disease, Monash Institute of Reproduction and DevelopmentMonash UniversityClaytonAustralia
  2. 2.Department of Anatomy and Cell BiologyMonash UniversityAustralia
  3. 3.The Prostate CentreJack Bell Research InstituteVancouverCanada
  4. 4.Bernard O’Brien Institute of MicrosurgeryFitzroyAustralia

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