Histochemistry and Cell Biology

, Volume 122, Issue 4, pp 277–291

Assembly of the phagocyte NADPH oxidase

Review

DOI: 10.1007/s00418-004-0679-8

Cite this article as:
Nauseef, W.M. Histochem Cell Biol (2004) 122: 277. doi:10.1007/s00418-004-0679-8

Abstract

Stimulated phagocytes undergo a burst in respiration whereby molecular oxygen is converted to superoxide anion through the action of an NADPH-dependent oxidase. The multicomponent phagocyte oxidase is unassembled and inactive in resting cells but assembles at the plasma or phagosomal membrane upon phagocyte activation. Oxidase components include flavocytochrome b558, an integral membrane heterodimer comprised of gp91phox and p22phox, and three cytosolic proteins, p47phox, p67phox, and Rac1 or Rac2, depending on the species and phagocytic cell. In a sense, the phagocyte oxidase is spatially regulated, with critical elements segregated in the membrane and cytosol but ready to undergo nearly immediate assembly and activation in response to stimulation. To achieve such spatial regulation, the individual components in the resting phagocyte adopt conformations that mask potentially interactive structural domains that might mediate productive intermolecular associations and oxidase assembly. In response to stimulation, post-translational modifications of the oxidase components release these constraints and thereby render potential interfaces accessible and interactive, resulting in translocation of the cytosolic elements to the membrane where the functional oxidase is assembled and active. This review summarizes data on the structural features of the phagocyte oxidase components and on the agonist-dependent conformational rearrangements that contribute to oxidase assembly and activation.

Keywords

NADPH oxidaseRespiratory burstNeutrophilsPhagocytesOxidase assembly

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  1. 1.Inflammation Program and Department of Medicine, Roy J. and Lucille A. Carver College of MedicineUniversity of IowaCoralvilleUSA
  2. 2.Department of Veterans AffairsIowa City VA Medical CenterIowa CityUSA