Original Paper

Histochemistry and Cell Biology

, Volume 121, Issue 3, pp 201-207

First online:

Localization of the Na+-d-glucose cotransporter SGLT1 in the blood-brain barrier

  • Katrin ElfeberAffiliated withInstitute of Anatomy and Cell Biology, University Würzburg
  • , Alwin KöhlerAffiliated withInstitute of Anatomy and Cell Biology, University Würzburg
  • , Michael LutzenburgAffiliated withClinic of Neurology, University Düsseldorf
  • , Christina OsswaldAffiliated withInstitute of Anatomy and Cell Biology, University Würzburg
  • , Hans-Joachim GallaAffiliated withInstitute of Biochemistry
  • , Otto W. WitteAffiliated withClinic of Neurology, University Düsseldorf
  • , Hermann KoepsellAffiliated withInstitute of Anatomy and Cell Biology, University Würzburg Email author 

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Abstract

Immunoreactivity of the Na+-d-glucose cotransporter SGLT1 was demonstrated in intracerebral capillaries of rat and pig. Immunostaining suggested that SGLT1 is located in the luminal membrane of the endothelial cells and in intracellular vesicles. Using in situ hybridization, SGLT1 mRNA was not detectable in intracerebral capillaries of non-treated or sham-operated Wistar rats. However, 1 day after a transient occlusion of the right middle cerebral artery, SGLT1 mRNA was detected in capillaries of both brain hemispheres. Expression of SGLT1 was also demonstrated in primary cultures of capillary endothelial cells from pig using polymerase chain reaction after reverse transcription and western blotting. The data suggest that SGLT1 participates in transport of d-glucose across the blood-brain barrier and is upregulated after brain ischemia and reperfusion.

Keywords

Sugar transport Sodium d-glucose cotransporter SGLT1 Blood-brain barrier Brain ischemia