Histochemistry and Cell Biology

, Volume 116, Issue 5, pp 453–462

Immunolocalization of hepatocyte growth factor and its receptor (c-Met) during mouse liver development

  • Kiyoshi S. Ishikawa
  • Tohru Masui
  • Katsutoshi Ishikawa
  • Nobuyoshi Shiojiri
Original Paper

DOI: 10.1007/s00418-001-0342-6

Cite this article as:
Ishikawa, K.S., Masui, T., Ishikawa, K. et al. Histochem Cell Biol (2001) 116: 453. doi:10.1007/s00418-001-0342-6

Abstract.

Although hepatocyte growth factor (HGF) was discovered as a potent hepatotrophic factor responsible for liver regeneration and may involve some organ development in embryogenesis, it remains to be revealed what roles HGF plays in liver development. The present study was undertaken to determine which cells express HGF and its receptor c-Met and when c-Met is activated in mouse liver development by using immunoblotting and immunohistochemical techniques. HGF was detected in hepatocytes and non-parenchymal cells, including biliary epithelial cells, periportal connective tissue cells, megakaryocytes, endothelial cells, and sinusoidal cells, throughout liver development. Positive HGF immunostaining in hepatocytes increased during postnatal development, and reached the maximal level in the adult stage. c-Met protein was also expressed in hepatocytes throughout liver development, but maximal staining was obtained in 1- or 2-week-old livers. Phosphorylation of tyrosine residues in the c-Met β chain also occurred in these stages. These results suggest that HGF signaling is implicated in hepatocyte growth during postnatal liver development, and its action could be in a paracrine mode; HGF produced by non-parenchymal cells such as sinusoidal cells acts on hepatocytes expressing c-Met receptors. Positive immunostaining in adult and postnatal hepatocytes may be derived from their blood clearance of HGF.

HGF c-Met Hepatocytes Growth Mouse

Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • Kiyoshi S. Ishikawa
    • 1
  • Tohru Masui
    • 2
  • Katsutoshi Ishikawa
    • 1
  • Nobuyoshi Shiojiri
    • 1
  1. 1.Department of Biology, Faculty of Science, Shizuoka University, Oya 836, Shizuoka 422-8529, JapanJapan
  2. 2.Cell Bank, Division of Genetics and Mutagenesis, National Institute of Health Sciences, Kamiyoga, Setagaya-ku, Tokyo, JapanJapan