Effect of intravitreal plasmin on vitreous removal through a 25-gauge cutting system in the rabbit in vivo
- Martin HermelAffiliated withDepartment of Ophthalmology, RWTH Aachen University Email author
- , Jonathan PrennerAffiliated withAssociated Retinal Consultants
- , Motaz AlabdulrazzakAffiliated withAssociated Retinal Consultants
- , Wendy DaileyAffiliated withNuVue Technologies
- , Michael HartzerAffiliated withNuVue Technologies
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Intravitreal plasmin creates a posterior vitreous detachment, but may also liquefy the vitreous. This study measures the rate of vitreous removal from rabbit eyes after plasmin injection in vivo.
Intravitreal injections of 150 IU hyaluronidase (n = 5), 0.5 activity units (AU, n = 6) or 0.9 AU of streptokinase-activated human plasmin (n = four groups of 6) in 0.1 ml were performed in rabbits, the fellow eyes received 0.1 ml BSS. After 30 min (hyaluronidase), 30 min, 4 h, 12 h or 24 h (0.9 AU plasmin) or 24 h (0.5 AU plasmin), 1 ml of vitreous was removed from each eye without infusion, using a 25-gauge cutter and a standardized protocol. Animals were sacrificed after surgery.
Compared to fellow eyes, the average rate of vitreous removal was increased by hyaluronidase by 68.9 ± 6.3% (p < 0.05) and by 0.5 AU plasmin (24 h) by 26.8 ± 3.3% (p < 0.05). 0.9 AU of plasmin increased removal rates by 0.8 ± 10% (n.s.), 15.4 ± 6.3% (p < 0.05), 40.3 ± 3.1% (p < 0.05), and 71.9 ± 32.4% (p < 0.05) after 30 min, 4 h, 12 h and 24 h incubation respectively. The ratios of removal rates of treated/control eyes in the 0.9 AU groups showed a linear correlation with incubation time (r = 0.783, p < 0.0001).
Intravitreal plasmin increases the rate of vitreous removal in rabbits.
KeywordsEnzyme administration and dosage Plasmin Plasmin drug effects Plasmin therapeutic use Vitrectomy Vitrectomy methods Vitreous body drug effects Vitreous body surgery
- Effect of intravitreal plasmin on vitreous removal through a 25-gauge cutting system in the rabbit in vivo
Graefe's Archive for Clinical and Experimental Ophthalmology
Volume 247, Issue 3 , pp 331-334
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- Enzyme administration and dosage
- Plasmin drug effects
- Plasmin therapeutic use
- Vitrectomy methods
- Vitreous body drug effects
- Vitreous body surgery
- Industry Sectors
- Author Affiliations
- 1. Department of Ophthalmology, RWTH Aachen University, Pauwelsstrasse 30, 52057, Aachen, Germany
- 3. Associated Retinal Consultants, Royal Oak, MI, USA
- 2. NuVue Technologies, Keene, NH, USA