Graefe's Archive for Clinical and Experimental Ophthalmology

, Volume 245, Issue 10, pp 1549–1557

CD4+CD25+Tregs express an increased LAG-3 and CTLA-4 in anterior chamber-associated immune deviation

  • Xuefei Zhu
  • Peizeng Yang
  • Hongyan Zhou
  • Bing Li
  • Xiangkun Huang
  • Qianli Meng
  • Li Wang
  • Aize Kijlstra
Laboratory Investigation

DOI: 10.1007/s00417-007-0591-8

Cite this article as:
Zhu, X., Yang, P., Zhou, H. et al. Graefes Arch Clin Exp Ophthalmol (2007) 245: 1549. doi:10.1007/s00417-007-0591-8

Abstract

Background

Regulatory CD4+CD25+ T cells have been proven to be essential for maintenance of peripheral tolerance and autoimmune diseases. ACAID is a model of immune privilege in the eye. Relatively little is known about the role and phenotype of these regulatory CD4+CD25+ T cells in ACAID.

Methods

Injection of OVA into the anterior chamber of BALB/C mice was performed to induce ACAID. The frequencies of splenic CD4+CD25+ Tregs and the expression of CTLA-4 and LAG-3 on these cells were determined by flow cytometry. Magnetic cell sorting was used to isolate CD4+CD25+ and CD4+CD25T cells. The function of CD4+CD25+ T cells was detected by in vitro immunosuppression assays and in vivo adoptive transfer.

Results

ACAID was successfully induced following an i.c. injection of OVA. Frequencies of CD4+CD25+ and Tregs were significantly increased in ACAID mice as compared to those in controls. The CD4+CD25+ T cells stimulated with OVA in ACAID mice showed a stronger suppressive ability in vitro than those seen in non-ACAID mice. CD4+CD25+ T cells from ACAID mice, but not from non-ACAID mice, were able to suppress DTH responses in an antigen-specific manner following adoptive transfer. The frequencies of CTLA-4 or LAG-3 on Tregs in ACAID mice were higher as compared with those in naive mice.

Conclusion

Splenic CD4+CD25+Foxp3+T cells expressing CTLA4 and LAG3 play an important role in the induction of ACAID.

Keywords

Regulatory T cellsImmune regulationFoxp3ACAID

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Xuefei Zhu
    • 1
  • Peizeng Yang
    • 1
  • Hongyan Zhou
    • 1
  • Bing Li
    • 1
  • Xiangkun Huang
    • 1
  • Qianli Meng
    • 1
  • Li Wang
    • 1
  • Aize Kijlstra
    • 2
    • 3
  1. 1.State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Uveitis Study CenterSun Yat-sen UniversityGuangzhouPeople’s Republic of China
  2. 2.Animal Sciences Group van Wageningen UR Divisie VeehouderijLelystadThe Netherlands
  3. 3.Department Ophthalmology, Eye Research Institute MaastrichtUniversity of MaastrichtMaastrichtThe Netherlands