Effects of direct intravitreal dopamine injections on the development of lid-suture induced myopia in rabbits

  • Qianying Gao
  • Quan Liu
  • Ping Ma
  • XingWu Zhong
  • Junshu Wu
  • Jian Ge
Laboratory Investigation

DOI: 10.1007/s00417-006-0254-1

Cite this article as:
Gao, Q., Liu, Q., Ma, P. et al. Graefe's Arch Clin Exp Ophthalmo (2006) 244: 1329. doi:10.1007/s00417-006-0254-1
  • 222 Downloads

Abstract

Background

Dopamine (DA) storage and release are reduced in form deprivation myopia (FDM) in a wide range of species, from chicks to primates. FDM can be prevented by treatment with DA agonists such as apomorphine, and paradoxically by the dopamine neurotoxin 6-hydroxydopamine. In this study, we increased the DA levels by direct intravitreal DA injections to learn if FDM can be suppressed in a rabbit model.

Methods

Seven-day-old rabbits were deprived of pattern vision by the suturing the right eyelids after natural eye opening. In the first group (FD, n=20), the right eye received form deprivation (FD) alone. In the second group (DA-FD, n=16), the deprived eye of 7-day-old rabbits received four intravitreal injections of 20 μg dopamine every 5 days. In the third group (saline-FD, n=16), the deprived eye received saline injections with the same schedule. The contralateral eye remained untreated as a control. At the end of the 8-week deprivation period, the effects of DA on refractive error, corneal curvature and ocular dimensions were assessed by streak retinoscopy, keratometry and A-scan ultrasonography, respectively.

Results

Eight weeks of FDM induced a myopic shift of −2.70±0.87 D (n=20) in treated eyes compared with contralateral eyes. The major structural correlate of the myopia appeared to be elongation of the vitreous chamber (0.7±0.3 mm, n=20) and axial elongation (0.9±0.3 mm, n=20), respectively. Repeated intravitreal injections of DA fully prevented the myopic shift (−0.06±0.37 D), elongation of the vitreous chamber (0.1±0.3 mm, n=16) and axial elongation (0.3±0.2 mm, n=16) due to lid suture, whereas saline injections had slight effect.

Conclusions

FD by suturing eyelids is an effective technique to induce a significant myopic shift, vitreous chamber and axial elongation in rabbits as a model of myopia development. These changes associated with FD were retarded by intravitreal injections of DA.

Keywords

Form deprivation myopia Rabbit Dopamine 

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Qianying Gao
    • 1
  • Quan Liu
    • 1
  • Ping Ma
    • 1
  • XingWu Zhong
    • 1
  • Junshu Wu
    • 1
  • Jian Ge
    • 1
  1. 1.Key Laboratory of Ophthalmology, Ministry of EducationZhongshan Ophthalmic CenterGuangzhouPeople’s Republic of China

Personalised recommendations