Graefe's Archive for Clinical and Experimental Ophthalmology

, Volume 244, Issue 8, pp 1003–1009

Neuroprotective effect of latanoprost on rat retinal ganglion cells

  • Hideyo Kudo
  • Toru Nakazawa
  • Masahiko Shimura
  • Hidetoshi Takahashi
  • Nobuo Fuse
  • Kenji Kashiwagi
  • Makoto Tamai
Laboratory Investigation

DOI: 10.1007/s00417-005-0215-0

Cite this article as:
Kudo, H., Nakazawa, T., Shimura, M. et al. Graefe's Arch Clin Exp Ophthalmo (2006) 244: 1003. doi:10.1007/s00417-005-0215-0
  • 156 Downloads

Abstract

Background

To investigate the neuroprotective effect of intravitreal administration of latanoprost on retinal ganglion cell (RGC) damage induced by N-methyl-D-aspartic acid (NMDA) or optic nerve axotomy.

Methods

Using Sprague-Dawley rats, retinal ganglion cell damage was induced by either intravitreal administration of NMDA or optic nerve axotomy. Latanoprost at doses of 0.03, 0.3, 3, 30 and 300 pmol was administered intravitreally before NMDA injection or optic nerve axotomy. Retinal damage was evaluated by counting the number of surviving RGCs retrogradely labeled with fluorogold under the microscope.

Results

Seven days after the NMDA injury, the number of surviving RGCs was significantly increased at doses of more than 30 pmol atanoprost (846±178 cells/mm2 P=0.0166) compared with vehicle control (556±122 cells/mm2). Ten days after the optic nerve axotomy, the number of surviving RGC was significantly increased even at a dose of 0.3 pmol (815±239 cells/mm2, P=0.0359) compared with control (462±75 cells/mm2).

Conclusions

Intravitreal administration of latanoprost has a neuroprotective effect on rat RGC damage induced by either NMDA or optic nerve axotomy, while its pharmacological features are different.

Keywords

NeuroprotectionRetinal ganglion cellAxotomyGlaucomaLatanoprostN-Methyl-D-aspartic acid

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Hideyo Kudo
    • 1
  • Toru Nakazawa
    • 1
  • Masahiko Shimura
    • 1
    • 2
  • Hidetoshi Takahashi
    • 1
  • Nobuo Fuse
    • 1
  • Kenji Kashiwagi
    • 3
  • Makoto Tamai
    • 1
  1. 1.Department of OphthalmologyTohoku University School of MedicineSendaiJapan
  2. 2.Department of OphthalmologyNTT East Japan Tohoku HospitalSendaiJapan
  3. 3.Department of Ophthalmology, University of YamanashiFaculty of MedicineTamahoJapan