Laboratory Investigation

Graefe's Archive for Clinical and Experimental Ophthalmology

, Volume 244, Issue 1, pp 104-108

First online:

Immunohistochemical localization of advanced glycation end products in pinguecula

  • Yuichi KajiAffiliated withDepartment of Ophthalmology, Tsukuba University Institute of Clinical Medicine Email author 
  • , Tetsuro OshikaAffiliated withDepartment of Ophthalmology, Tsukuba University Institute of Clinical Medicine
  • , Shiro AmanoAffiliated withDepartment of Ophthalmology, University of Tokyo Graduate School of Medicine
  • , Fumiki OkamotoAffiliated withDepartment of Ophthalmology, Tsukuba University Institute of Clinical Medicine
  • , Wakako KoitoAffiliated withDepartment of Medical Biochemistry, Graduate School of Medical and Pharmaceutical Sciences, Kumamoto University
  • , Seikoh HoriuchiAffiliated withDepartment of Medical Biochemistry, Graduate School of Medical and Pharmaceutical Sciences, Kumamoto University

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Abstract

Background

Advanced glycation end products (AGEs) are known to be deposited in the target organ of ageing. In addition, the deposition of AGEs accelerate the process of ageing. We investigated the immunohistochemical localization of AGEs in pinguecula, one of the ocular changes related with ageing process.

Methods

Surgical specimens of conjunctiva with or without pinguecula were prepared from nine patients, respectively. Immunohistochemical localization of AGEs was investigated using monoclonal antibodies to\({\text{N}}^{{\text{ $ \varepsilon $ }}} \)-(carboxymethyl)lysine, pentosidine, imidazolone, and pyrraline.

Results

Moderate to strong immunoreactivities to AGEs were detected in the subepithelial amorphous deposits of all the surgical specimens with pinguecula. In contrast, no or weak immunoreactivities to AGEs were detected in the surgical specimens without pinguecula.

Conclusions

Pinguecula is an aggregation of AGEs-modified proteins. The presence of pinguecula would be an index of local irradiation of ultraviolet rays and decreased antioxidant activities.

Keywords

Advanced glycation Pinguecula CML Ageing