Short communication

International Journal of Legal Medicine

, Volume 115, Issue 2, pp 109-117

Y-Chromosome multiplexes and their potential for the DNA profiling of Koreans

  • D. J. ShinAffiliated withDepartment of Biology, Dankook University, Cheonan 330-714, Korea e-mail: wookkim@anseo.dankook.ac.kr, Tel.: +82-41-5503441, Fax: +82-41-5503441
  • , H. J. JinAffiliated withDepartment of Biology, Dankook University, Cheonan 330-714, Korea e-mail: wookkim@anseo.dankook.ac.kr, Tel.: +82-41-5503441, Fax: +82-41-5503441
  • , K. D. KwakAffiliated withDepartment of Biology, Dankook University, Cheonan 330-714, Korea e-mail: wookkim@anseo.dankook.ac.kr, Tel.: +82-41-5503441, Fax: +82-41-5503441
  • , J. W. ChoiAffiliated withDepartment of Biology, Dankook University, Cheonan 330-714, Korea e-mail: wookkim@anseo.dankook.ac.kr, Tel.: +82-41-5503441, Fax: +82-41-5503441
  • , M. S. HanAffiliated withDNA Analysis Section, National Institute of Scientific Investigation, Seoul 158-097, Korea
  • , P. W. KangAffiliated withDNA Analysis Section, National Institute of Scientific Investigation, Seoul 158-097, Korea
  • , S. K. ChoiAffiliated withDNA Analysis Section, National Institute of Scientific Investigation, Seoul 158-097, Korea
  • , W. KimAffiliated withDepartment of Biology, Dankook University, Cheonan 330-714, Korea e-mail: wookkim@anseo.dankook.ac.kr, Tel.: +82-41-5503441, Fax: +82-41-5503441

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Abstract

We have developed four multiplex genotyping systems (GeneKin Y-STR multiplexes) using silver staining with allelic ladders for ten Y-chromosome STR markers (DYS19, DYS385, DYS388, DYS389I/II, DYS390, DYS391, DYS392, DYS393 and DXYS156Y), with a view towards the application of rapid and simple genotyping assay methods for DNA profiling. The GeneKin Y-STR multiplexes developed have followed the published nomenclature and ISFG guidelines for STR analysis. Allele and haplotype frequencies at these Y-STRs loci were analysed by PCR amplification using the GeneKin Y-STR multiplexes, followed by denaturing polyacrylamide gel electrophoresis in 316 unrelated males in the Korean population. A total of 295 different haplotypes were found, 279 of them being unique. Gene diversity ranged from 0.4026 at DYS391 to 0.9606 at DYS385. The haplotype diversity value (which is the same as the discrimination index) calculated from all ten loci combined was 0.9995, which is informative. Our results revealed that a set of ten Y-STRs can discriminate between most of the male individuals in the Korean population (discrimination capacity: 93.35%). The Y-STR multiplexes thus provide useful information for forensic analysis and paternity tests and can also be of great benefit for providing information not normally available from autosomal DNA systems.

Keywords Allelic ladders DNA profiling Koreans STRs Y-chromosome haplotypes