International Journal of Legal Medicine

, Volume 113, Issue 3, pp 189–192

Y-chromosomal STR haplotypes in a population sample from Bavaria

Authors

  • K. Anslinger
    • Institute of Legal Medicine, Ludwig-Maximilians-University of Munich, Frauenlobstrasse 7 a, D-80337 Munich, Germany Tel. +49-89-51605121, Fax +49-89-51605144
  • W. Keil
    • Institute of Legal Medicine, Ludwig-Maximilians-University of Munich, Frauenlobstrasse 7 a, D-80337 Munich, Germany Tel. +49-89-51605121, Fax +49-89-51605144
  • G. Weichhold
    • Institute of Legal Medicine, Ludwig-Maximilians-University of Munich, Frauenlobstrasse 7 a, D-80337 Munich, Germany Tel. +49-89-51605121, Fax +49-89-51605144
  • W. Eisenmenger
    • Institute of Legal Medicine, Ludwig-Maximilians-University of Munich, Frauenlobstrasse 7 a, D-80337 Munich, Germany Tel. +49-89-51605121, Fax +49-89-51605144
Short communication

DOI: 10.1007/s004140050296

Cite this article as:
Anslinger, K., Keil, W., Weichhold, G. et al. Int J Leg Med (2000) 113: 189. doi:10.1007/s004140050296

Abstract

The seven Y-chromosomal STRs DYS19, DYS385 I/II, DYS389 I/II, DYS390, DYS391, DYS392 and DYS393 were amplified using two multiplex PCRs. The optimization of the PCR conditions led to reliable and sensitive systems. Co-amplification of the amelogenin locus was possible in both multiplex systems. In a population sample of 151 Bavarian males, a gene diversity of 0.99 was observed. Sensitivity studies revealed a detection limit of 50 pg DNA per 25 μl reaction volume. PCR experiments with combinations of male/male and male/female DNA showed that in male/male mixtures, the minor component could be detected up to a ratio of 1:15, whereas in male/female mixtures the male component could be found in a higher ratio up to 1: 60.

Key words Y-chromosomeSTRsMultiplexY haplotypeMixtures

Copyright information

© Springer-Verlag Berlin Heidelberg 2000