International Journal of Legal Medicine

, Volume 112, Issue 2, pp 124–131

Hydroxyproline interference during the gas chromatographic analysis of D/L aspartic acid in human dentine

  • E. R. Waite
  • M. J. Collins
  • A. C. T. van Duin
Technical note

DOI: 10.1007/s004140050214

Cite this article as:
Waite, E., Collins, M. & van Duin, A. Int J Leg Med (1999) 112: 124. doi:10.1007/s004140050214
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Abstract

The proportion of D- to L-enantiomers of aspartic acid in metabolically isolated proteins has been used by forensic scientists to estimate age at death. We have demonstrated the interference of a derivative of hydroxyproline (N-TFA isopropyl Hyp ester) with the N-TFA isopropyl L-Aspartic (Asp) acid ester during gas chromatography of amino acids. This has serious implications for the accurate quantification of the D- to L-Asp ratio extracted from collagenous proteins. Having demonstrated the potential for this co-elution in amino acid standards, acid-soluble dentine proteins and non-mineralised collagen, we argue that this problem can be overcome either by high resolution separation or by analysis of the (Hyp-poor) non-collagenous protein fraction.

Key words HydroxyprolineD/L Aspartic acidN-TFA isopropyl ester derivativesGas chromatographyCollagen

Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • E. R. Waite
    • 1
  • M. J. Collins
    • 1
  • A. C. T. van Duin
    • 1
  1. 1.Fossil Fuels and Environmental Geochemistry (NRG), Drummond Building, University of Newcastle upon Tyne, University of Newcastle upon Tyne, NE1 7RU, UK email: emma.waite@ncl.ac.uk Fax +44-191-222-5431GB