Short Communication

International Journal of Legal Medicine

, Volume 127, Issue 2, pp 359-362

Open Access This content is freely available online to anyone, anywhere at any time.

Genetic investigation of biological materials from patients after stem cell transplantation based on autosomal as well as Y-chromosomal markers

  • Renata JacewiczAffiliated withForensic Genetics Laboratory, Department of Forensic Medicine, Medical University of Lodz Email author 
  • , Krzysztof LewandowskiAffiliated withDepartment of Hematology, Poznan University of Medical Sciences
  • , Joanna Rupa-MatysekAffiliated withDepartment of Hematology, Poznan University of Medical Sciences
  • , Maciej JedrzejczykAffiliated withDepartment of Forensic Court and Insurance Certification, Medical University of Lodz
  • , Mieczysław KomarnickiAffiliated withDepartment of Hematology, Poznan University of Medical Sciences
  • , Jarosław BerentAffiliated withForensic Genetics Laboratory, Department of Forensic Medicine, Medical University of Lodz

Abstract

The authors presented the results of DNA polymorphism investigation of blood, buccal swabs and hair follicles originating from patients after allogeneic hematopoietic stem cell transplantation. The real-time and multiplex assays based on polymerase chain reaction within the range of autosomal as well as Y-chromosomal markers were applied to assess the possible dangers arising from investigation of these materials in forensic genetics. The results revealed that not only post-transplant blood and buccal swab, but also recipient hair, up to now regarded as devoid of any donor’s cells, do not constitute entirely safe material for forensic purposes. Their analysis can lead to the false identification of gender or male haplotype. The investigation of sex-determining region Y and Y-chromosome short tandem repeats performed in female recipients with male donors resulted in the designation of donor’s DNA in hair cells as well as in blood and buccal swabs. Therefore, biological stains gathered from crime scenes should not be analysed exclusively based on the investigation of male-specific markers.

Keywords

Stem cell transplantation Forensic genetics DNA analysis Y chromosome markers