Original Article

International Journal of Legal Medicine

, Volume 123, Issue 4, pp 315-325

Pigment phenotype and biogeographical ancestry from ancient skeletal remains: inferences from multiplexed autosomal SNP analysis

  • Caroline BouakazeAffiliated withLaboratoire d’Anthropologie Moléculaire, Institut de Médecine Légale Email author 
  • , Christine KeyserAffiliated withLaboratoire d’Anthropologie Moléculaire, Institut de Médecine LégaleLaboratoire d’Anthropobiologie, FRE 2960 CNRS, Université Paul Sabatier
  • , Eric CrubézyAffiliated withLaboratoire d’Anthropobiologie, FRE 2960 CNRS, Université Paul Sabatier
  • , Daniel MontagnonAffiliated withLaboratoire d’Anthropologie Moléculaire, Institut de Médecine Légale
  • , Bertrand LudesAffiliated withLaboratoire d’Anthropologie Moléculaire, Institut de Médecine LégaleLaboratoire d’Anthropobiologie, FRE 2960 CNRS, Université Paul Sabatier

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Abstract

In the present study, a multiplexed genotyping assay for ten single nucleotide polymorphisms (SNPs) located within six pigmentation candidate genes was developed on modern biological samples and applied to DNA retrieved from 25 archeological human remains from southern central Siberia dating from the Bronze and Iron Ages. SNP genotyping was successful for the majority of ancient samples and revealed that most probably had typical European pigment features, i.e., blue or green eye color, light hair color and skin type, and were likely of European individual ancestry. To our knowledge, this study reports for the first time the multiplexed typing of autosomal SNPs on aged and degraded DNA. By providing valuable information on pigment traits of an individual and allowing individual biogeographical ancestry estimation, autosomal SNP typing can improve ancient DNA studies and aid human identification in some forensic casework situations when used to complement conventional molecular markers.

Keywords

Autosomal SNPs Pigmentation Ancestry Ancient DNA SNaPshot