International Journal of Legal Medicine

, Volume 121, Issue 5, pp 423–427

Two universal primer sets for species identification among vertebrates

  • Takashi Kitano
  • Kazuo Umetsu
  • Wei Tian
  • Motoki Osawa
Short Communication

DOI: 10.1007/s00414-006-0113-y

Cite this article as:
Kitano, T., Umetsu, K., Tian, W. et al. Int J Legal Med (2007) 121: 423. doi:10.1007/s00414-006-0113-y

Abstract

The aim of this study was to develop a simple method using universal primers for species identification based on direct PCR sequencing. Two primer sets were designed based on the conserved regions of the 12S and 16S rRNA loci detected by the comprehensive sequence comparison among 30 mammalian whole mitochondrial genomes. In humans, the expected sizes of PCR products of the 12S and 16S rRNAs were 215 and 244  bp, respectively. Both primer sets successfully amplified the expected PCR products from various kinds of vertebrates including mammals, birds, reptiles, amphibians, and fish, and the sequenced segments contained sufficient nucleotide differences to identify each animal species. A case example of the identification of a piece of buried bone of unknown species is presented, and the species was identified as a pig by this method.

Keywords

Mitochondrial DNA12S rRNA16S rRNASpecies identificationVertebrates

Supplementary material

414_2006_113_MOESM1_ESM.doc (70 kb)
Supplementary Table(DOC 71 kb)

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Takashi Kitano
    • 1
  • Kazuo Umetsu
    • 1
  • Wei Tian
    • 1
  • Motoki Osawa
    • 2
  1. 1.Department of Experimental and Forensic Pathology, Faculty of MedicineYamagata UniversityYamagataJapan
  2. 2.Department of Forensic MedicineTokai University School of MedicineIseharaJapan