Is the amelogenin gene reliable for gender identification in forensic casework and prenatal diagnosis?
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In humans, the amelogenin gene is present on both the X and the Y chromosomes. However, there are size differences in this gene between these chromosomes, which have been utilised for sexing in forensic casework and prenatal diagnosis. Our study using the AmpFl STR Profiler Plus kit, showed a deletion of Y chromosome-specific amelogenin in five Indian males (1.85%). We propose to call them “deleted-amelogenin males” (DAMs), who but for the detection of the presence of other Y-specific markers (e.g. SRY, STR and 50f2) would have been identified as females. Considering the consequences of the result obtained only using the amelogenin marker, we suggest the use of additional Y chromosome markers for unambiguous gender identification.
- Bailey DMD, Affara NA, Ferguson-Smith M (1992) The X-Y homologous gene amelogenin maps to the short arms of both the X and the Y chromosomes and is highly conserved in primates. Genomics 14:203–205 CrossRef
- Haas-Rochholz H, Weiler G (1997) Additional primer sets for an amelogenin gene PCR-base DNA-sex test. Int J Legal Med 110:312–315 CrossRef
- Han GR, Lee YW, Lee HL, Kim SM, Ku TW, Kang IH, Lee HS, Hwang JJ (2000) A Korean population study of the nine STR loci FGA, vWA, D3S1358, D18S11, D21S11, D8S1179, D13S317 and D5S818. Int J Legal Med 114:41–44 CrossRef
- Jobling MA (1994) A survey of long-range DNA polymorphisms on the human Y chromosome. Hum Mol Genet 3:107–114 CrossRef
- Mannucci A, Sullivan KM, Ivanov PL, Gill P (1994) Forensic application of a rapid and quantitative DNA sex test by amplification of the X-Y homologous gene amelogenin. Int J Legal Med 106:190–193 CrossRef
- Pawlowski R, Maciejewska A (2000) Forensic validation of a multiplex containing nine STRs — population genetics in northern Poland. Int J Legal Med 114:45–49 CrossRef
- Santos FR, Pandya A, Tyler-Smith C (1998) Reliability of DNA-based sex tests. Nat Genet 18:103 CrossRef
- Singh L, Pathak NH, Rachel AJ, Thangaraj K (1999) Snake’s eye view of Adam and Eve. In: Gupta SK (ed) Reproductive immunology. Narosa Publishing. New Delhi, pp 132–148
- Steinlechner M, Berger B, Niederstätter H, Parson W (2002) Rare failures in the amelogenin sex test. Int J Legal Med 116:117–120 CrossRef
- Thangaraj K, Ramana GV, Singh L (1999) Y-chromosome and mitochondrial DNA polymorphisms in Indian populations. Electrophoresis 20:1743–1747 CrossRef
- Is the amelogenin gene reliable for gender identification in forensic casework and prenatal diagnosis?
International Journal of Legal Medicine
Volume 116, Issue 2 , pp 121-123
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- Gender identification
- Y chromosome
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