Chromosoma

, Volume 107, Issue 6, pp 471–478

Regulation of tubulin polypeptides and microtubule function: Rki1p interacts with the β-tubulin binding protein Rbl2p

  • Adelle M. Smith
  • Julie E. Archer
  • Frank Solomon

DOI: 10.1007/s004120050331

Cite this article as:
Smith, A., Archer, J. & Solomon, F. Chromosoma (1998) 107: 471. doi:10.1007/s004120050331

Abstract.

Genetic analysis of microtubule functions in the yeast Saccharomyces cerevisiae suggests that cells manage the levels and activities of the tubulin polypeptides. These reactions may be involved in protein folding, formation of the heterodimer, and maintenance of the appropriate balance between α- and β-tubulin. One protein involved in these functions is Rbl2p, which forms a complex with β-tubulin. Here we describe the identification of a novel yeast gene, RKI1, that interacts genetically with RBL2. Deletion of rki1 causes conditional defects in microtubule assembly and cell growth. Rki1p can be isolated in a complex containing Rbl2p. The results support the existence of cellular mechanisms for regulating microtubule function through the tubulin polypeptides.

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Adelle M. Smith
    • 1
  • Julie E. Archer
    • 1
  • Frank Solomon
    • 1
  1. 1.Department of Biology and Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USAUS