Chromosoma

, Volume 106, Issue 6, pp 335–347

The localization of histone H3.3 in germ line chromatin of Drosophila males as established with a histone H3.3-specific antiserum

Authors

  • Anna Akhmanova
    • Department of Molecular and Developmental Genetics, Faculty of Sciences, Catholic University of Nijmegen, Nijmegen, The Netherlands
  • Koos Miedema
    • Department of Molecular and Developmental Genetics, Faculty of Sciences, Catholic University of Nijmegen, Nijmegen, The Netherlands
  • Yaxian Wang
    • Max Planck Guest Laboratory, Institute of Cell Biology, Academia Sinica, 320 Yo Yang Road, Shanghai, China
  • Mieke van Bruggen
    • Division of Nephrology, University Hospital, Nijmegen, The Netherlands
  • Jo H. M. Berden
    • Division of Nephrology, University Hospital, Nijmegen, The Netherlands
  • Evangelos N. Moudrianakis
    • Department of Biology, The Johns Hopkins University, Baltimore, USA
  • Wolfgang Hennig
    • Department of Molecular and Developmental Genetics, Faculty of Sciences, Catholic University of Nijmegen, Nijmegen, The Netherlands

DOI: 10.1007/s004120050255

Cite this article as:
Akhmanova, A., Miedema, K., Wang, Y. et al. Chromosoma (1997) 106: 335. doi:10.1007/s004120050255
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Abstract.

A rabbit antiserum, specific for the histone H3.3 replacement variant, was raised with the aid of a histone H3.3-specific peptide. Immuno blot experiments demonstrated the specificity of this polyclonal antiserum. In addition, we showed on immuno blots that two monoclonal antibodies isolated from mice with systemic lupus erythematosus (SLE) display strong reactivity with the H3.3 histone, but not with its replication-dependent counterparts. Our observations indicate that histone H3.3 might play a role as autoantigen in SLE. We used the histone H3.3-specific antiserum to characterize the germ line chromatin in cytological preparations of Drosophila testes, because our previous studies had shown that a histone H3.3-encoding gene is strongly expressed in the germ line of Drosophila males. The antiserum reacted with some of the lampbrush loops in spermatocytes and with chromatin of the postmeiotic germ cells of males. Our data indicate that histone H3.3 is not evenly distributed throughout the chromatin of germ cells, but is concentrated in distinct regions. Histone H3.3 disappears from the spermatid nuclei, along with the other core histones, during the late stages of spermatogenesis. In Drosophila polytene chromosomes, however, a rather uniform distribution of the histone H3.3 was observed. The possible role of histone H3.3 is discussed.

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© Springer-Verlag Berlin Heidelberg 1997