Chromosoma

, Volume 106, Issue 3, pp 136–148

Yeast silencers create domains of nuclease-resistant chromatin in an SIR4-dependent manner

  • Susan K. Reimer
  • Andrew R. Buchman

DOI: 10.1007/s004120050233

Cite this article as:
Reimer, S. & Buchman, A. Chromosoma (1997) 106: 136. doi:10.1007/s004120050233

Abstract.

Previous analysis of the repression of the silent mating type loci in Saccharomyces cerevisiae has linked the mechanism of silencing to the formation of a chromatin domain at the silenced loci. In this study, a TRP1 reporter gene was used to examine changes in chromatin structure in a neutral environment. This enabled the chromatin structure organized by yeast silencers to be compared directly with changes effected by the yeast α2 repressor. It was found that silencers mediate the formation of lengthy nuclease-resistant domains on the DNA, rather than specifically positioning nucleosomes over promoter regions as the α2 repressor does. Silencing at the TRP1 reporter gene closely resembled silencing at the HMR and HML loci. Repression of the test gene was optimal when two silencers flanking the reporter gene were used, mimicking the situation at the silent loci. In addition, both repression of the reporter gene and the formation of nuclease-resistant chromatin domains was SIR4 dependent.

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • Susan K. Reimer
    • 1
  • Andrew R. Buchman
    • 1
  1. 1.Center for Gene Regulation, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USAUS