Chromosoma

, Volume 109, Issue 7, pp 482–489

Evolutionary conservation of kinetochore protein sequences in plants

  • Rogier ten Hoopen
  • Renate Manteuffel
  • Jaroslav Doležel
  • Ludmilla Malysheva
  • Ingo Schubert
Original Article

DOI: 10.1007/s004120000109

Cite this article as:
ten Hoopen, R., Manteuffel, R., Doležel, J. et al. Chromosoma (2000) 109: 482. doi:10.1007/s004120000109

Abstract.

The evolutionary conservation of structural/functional kinetochore proteins has been studied on isolated nuclei and pro-/metaphase chromosomes of mono- and dicot plants. The cross-reactivities of antibodies against human CENPC, CENPE and CENPF, and against maize CENPCa with the centromeric regions of mitotic chromosomes of Vicia faba and/or Hordeum vulgare are shown. Putative homologs of the kinetochore protein SKP1 (suppressor of kinetochore protein 1p of yeast) were found in both species and of CBF5p (centromere binding factor 5 of yeast) in barley. Antibodies against synthetic peptides derived from partial sequences encoding these proteins were produced and recognized the centromeric regions on mitotic chromosomes as detected by indirect immunofluorescence.

Copyright information

© Springer-Verlag 2000

Authors and Affiliations

  • Rogier ten Hoopen
    • 1
  • Renate Manteuffel
    • 1
  • Jaroslav Doležel
    • 2
  • Ludmilla Malysheva
    • 1
  • Ingo Schubert
    • 1
  1. 1.Institute of Plant Genetics and Crop Plant Research (IPK), 06466 Gatersleben, Germany
  2. 2.Institute of Experimental Botany, 77200 Olomouc, Czech Republic

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