Chromosoma

, Volume 117, Issue 5, pp 457–469

Plk1 regulates mitotic Aurora A function through βTrCP-dependent degradation of hBora

  • Eunice H. Y. Chan
  • Anna Santamaria
  • Herman H. W. Silljé
  • Erich A. Nigg
Research Article

DOI: 10.1007/s00412-008-0165-5

Cite this article as:
Chan, E.H.Y., Santamaria, A., Silljé, H.H.W. et al. Chromosoma (2008) 117: 457. doi:10.1007/s00412-008-0165-5

Abstract

Polo-like kinase 1 (Plk1) and Aurora A play key roles in centrosome maturation, spindle assembly, and chromosome segregation during cell division. Here we show that the functions of these kinases during early mitosis are coordinated through Bora, a partner of Aurora A first identified in Drosophila. Depletion of human Bora (hBora) results in spindle defects, accompanied by increased spindle recruitment of Aurora A and its partner TPX2. Conversely, hBora overexpression induces mislocalization of Aurora A and monopolar spindle formation, reminiscent of the phenotype seen in Plk1-depleted cells. Indeed, Plk1 regulates hBora. Following Cdk1-dependent recruitment, Plk1 triggers hBora destruction by phosphorylating a recognition site for \({\text{SCF}}^{{\text{ $ \beta $ - TrCP}}} \). Plk1 depletion or inhibition results in a massive accumulation of hBora, concomitant with displacement of Aurora A from spindle poles and impaired centrosome maturation, but remarkably, co-depletion of hBora partially restores Aurora A localization and bipolar spindle formation. This suggests that Plk1 controls Aurora A localization and function by regulating cellular levels of hBora.

Supplementary material

412_2008_165_MOESM1_ESM.doc (2.5 mb)
ESM 1 (DOC 70.5 KB)

Copyright information

© The Author(s) 2008

Authors and Affiliations

  • Eunice H. Y. Chan
    • 1
    • 2
  • Anna Santamaria
    • 1
  • Herman H. W. Silljé
    • 1
    • 3
  • Erich A. Nigg
    • 1
  1. 1.Department of Cell BiologyMax Planck Institute of BiochemistryMartinsriedGermany
  2. 2.Apoptosis and Proliferation Control Laboratory, Cancer Research UKLondon Research InstituteLondonUK
  3. 3.Kiadis PharmaGroningenThe Netherlands

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