Chromosoma

, Volume 113, Issue 7, pp 385–397

Novel components of human mitotic chromosomes identified by proteomic analysis of the chromosome scaffold fraction

  • Reto Gassmann
  • Alexander J. Henzing
  • William C. Earnshaw
Research Article

DOI: 10.1007/s00412-004-0326-0

Cite this article as:
Gassmann, R., Henzing, A.J. & Earnshaw, W.C. Chromosoma (2005) 113: 385. doi:10.1007/s00412-004-0326-0

Abstract

Chromosomal nonhistone proteins have important roles in mitotic chromosome formation and dynamics. In order to identify novel abundant proteins with a potential involvement in these processes, we initiated a proteomic screen of the chromosome scaffold fraction. This screen identified 79 proteins, 30 of which had not previously been described as components of mitotic chromosomes. Furthermore, half of these proteins had no documented function. We analyzed the cell-cycle dependent distribution of three uncharacterized proteins by expressing them as green fluorescent protein (GFP) fusions and showed that they associate with mitotic chromosomes in vivo. One of the proteins, nuclear protein p30, is a novel component of the inner centromere. Over-expression experiments indicated that p30 may have an active role in the formation of centromeric heterochromatin.

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Reto Gassmann
    • 1
  • Alexander J. Henzing
    • 1
    • 2
  • William C. Earnshaw
    • 1
  1. 1.Wellcome Trust Centre for Cell Biology, School of Biological SciencesUniversity of EdinburghEdinburghUK
  2. 2.Scottish Instrumentation & Resource Centre for Advanced Mass Spectrometry, School of ChemistryUniversity of EdinburghEdinburghUK

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