Radiation and Environmental Biophysics

, Volume 38, Issue 2, pp 133–138

Repair of cellular radiation damage in space under microgravity conditions

  • H. D. Pross
  • J. Kiefer
Original Paper

DOI: 10.1007/s004110050149

Cite this article as:
Pross, H. & Kiefer, J. Radiat Environ Biophys (1999) 38: 133. doi:10.1007/s004110050149

Abstract 

The influence of microgravity on the repair of x-ray-induced DNA double-strand breaks was studied in the temperature-conditional repair mutant rad54–3 of diploid yeast Saccharomyces cerevisiae. Cells were exposed on the ground and kept at a low temperature until microgravity conditions were achieved. In orbit, they were incubated at the permissive temperature to allow repair. Before re-entry they were again cooled down and kept at a low temperature until final analysis. The experiment, which was flown on the shuttle Atlantis on flight STS-76 (SMM-03), showed that repair of pre-formed DNA double-strand breaks in yeast is not impaired by microgravity.

Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • H. D. Pross
    • 1
  • J. Kiefer
    • 1
  1. 1.Strahlenzentrum der Justus-Liebig-Universität, Leihgesterner Weg 217, D-35392 Giessen, Germany e-mail: Juergen.Kiefer@strz.uni-giessen.de Tel.: +49-641-9915300 Fax: +49-641-9915009DE