Radiation and Environmental Biophysics

, Volume 52, Issue 3, pp 411–417

UV microspot irradiator at Columbia University

  • Alan W. Bigelow
  • Brian Ponnaiya
  • Kimara L. Targoff
  • David J. Brenner
Short Communication

DOI: 10.1007/s00411-013-0474-9

Cite this article as:
Bigelow, A.W., Ponnaiya, B., Targoff, K.L. et al. Radiat Environ Biophys (2013) 52: 411. doi:10.1007/s00411-013-0474-9
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Abstract

The Radiological Research Accelerator Facility at Columbia University has recently added a UV microspot irradiator to a microbeam irradiation platform. This UV microspot irradiator applies multiphoton excitation at the focal point of an incident laser as the source for cell damage, and with this approach, a single cell within a 3D sample can be targeted and exposed to damaging UV. The UV microspot’s ability to impart cellular damage within 3D is an advantage over all other microbeam techniques, which instead impart damage to numerous cells along microbeam tracks. This short communication is an overview, and a description of the UV microspot including the following applications and demonstrations of selective damage to live single cell targets: DNA damage foci formation, patterned irradiation, photoactivation, targeting of mitochondria, and targeting of individual cardiomyocytes in a live zebrafish embryo.

Keywords

DNA damage Microbeams UV 

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Alan W. Bigelow
    • 1
  • Brian Ponnaiya
    • 1
  • Kimara L. Targoff
    • 2
  • David J. Brenner
    • 1
  1. 1.Center for Radiological ResearchColumbia University Medical CenterNew YorkUSA
  2. 2.Division of Pediatric Cardiology, Department of Pediatrics, College of Physicians and SurgeonsColumbia UniversityNew YorkUSA