Radiation and Environmental Biophysics

, Volume 50, Issue 1, pp 125–134

Frozen human cells can record radiation damage accumulated during space flight: mutation induction and radioadaptation

Authors

    • The Institute of Physical and Chemical Research (RIKEN)
  • Masamitsu Honma
    • Division of Genetics and MutagenesisNational Institute of Health Sciences
  • Akihisa Takahashi
    • Biology LabNara Medical University
  • Katsunori Omori
    • Institute of Space and Astronautical ScienceJapan Aerospace Exploration Agency
  • Hiromi Suzuki
    • Japan Space Forum
  • Toru Shimazu
    • Japan Space Forum
  • Masaya Seki
    • Advanced Engineering Services
  • Toko Hashizume
    • Advanced Engineering Services
  • Akiko Ukai
    • Division of Genetics and MutagenesisNational Institute of Health Sciences
  • Kaoru Sugasawa
    • Biosignal Research CenterKobe University
  • Tomoko Abe
    • The Institute of Physical and Chemical Research (RIKEN)
  • Naoshi Dohmae
    • The Institute of Physical and Chemical Research (RIKEN)
  • Shuichi Enomoto
    • The Institute of Physical and Chemical Research (RIKEN)
  • Takeo Ohnishi
    • Biology LabNara Medical University
  • Alasdair Gordon
    • Baylor College of Medicine
  • Noriaki Ishioka
    • Institute of Space and Astronautical ScienceJapan Aerospace Exploration Agency
Original Paper

DOI: 10.1007/s00411-010-0348-3

Cite this article as:
Yatagai, F., Honma, M., Takahashi, A. et al. Radiat Environ Biophys (2011) 50: 125. doi:10.1007/s00411-010-0348-3

Abstract

To estimate the space-radiation effects separately from other space-environmental effects such as microgravity, frozen human lymphoblastoid TK6 cells were sent to the “Kibo” module of the International Space Station (ISS), preserved under frozen condition during the mission and finally recovered to Earth (after a total of 134 days flight, 72 mSv). Biological assays were performed on the cells recovered to Earth. We observed a tendency of increase (2.3-fold) in thymidine kinase deficient (TK) mutations over the ground control. Loss of heterozygosity (LOH) analysis on the mutants also demonstrated a tendency of increase in proportion of the large deletion (beyond the TK locus) events, 6/41 in the in-flight samples and 1/17 in the ground control. Furthermore, in-flight samples exhibited 48% of the ground-control level in TK mutation frequency upon exposure to a subsequent 2 Gy dose of X-rays, suggesting a tendency of radioadaptation when compared with the ground-control samples. The tendency of radioadaptation was also supported by the post-flight assays on DNA double-strand break repair: a 1.8- and 1.7-fold higher efficiency of in-flight samples compared to ground control via non-homologous end-joining and homologous recombination, respectively. These observations suggest that this system can be used as a biodosimeter, because DNA damage generated by space radiation is considered to be accumulated in the cells preserved frozen during the mission, Furthermore, this system is also suggested to be applicable for evaluating various cellular responses to low-dose space radiation, providing a better understanding of biological space-radiation effects as well as estimation of health influences of future space explores.

Copyright information

© Springer-Verlag 2010