Original Paper

Radiation and Environmental Biophysics

, Volume 50, Issue 1, pp 155-166

Low-dose irradiation causes rapid alterations to the proteome of the human endothelial cell line EA.hy926

  • Franka PluderAffiliated withInstitute of Radiation Biology, Helmholtz Zentrum München, German Research Center for Environmental Health
  • , Zarko BarjaktarovicAffiliated withInstitute of Radiation Biology, Helmholtz Zentrum München, German Research Center for Environmental Health
  • , Omid AzimzadehAffiliated withInstitute of Radiation Biology, Helmholtz Zentrum München, German Research Center for Environmental Health
  • , Simone MörtlAffiliated withInstitute of Radiation Biology, Helmholtz Zentrum München, German Research Center for Environmental Health
  • , Anne KrämerAffiliated withInstitute of Radiation Biology, Helmholtz Zentrum München, German Research Center for Environmental Health
  • , Sylvia SteiningerAffiliated withInstitute of Radiation Biology, Helmholtz Zentrum München, German Research Center for Environmental Health
  • , Hakan SariogluAffiliated withDepartment of Protein Science, Helmholtz Zentrum München, German Research Center for Environmental Health
  • , Dariusz LeszczynskiAffiliated withSTUK—Radiation and Nuclear Safety Authority
  • , Reetta NylundAffiliated withSTUK—Radiation and Nuclear Safety Authority
    • , Arvi HakanenAffiliated withSTUK—Radiation and Nuclear Safety Authority
    • , Arundhathi SriharshanAffiliated withDivision of Radiation Cytogenetics, Helmholtz Zentrum München, German Research Center for Environmental Health
    • , Michael J. AtkinsonAffiliated withInstitute of Radiation Biology, Helmholtz Zentrum München, German Research Center for Environmental HealthDepartment of Radiation Oncology, Klinikum Rechts der Isar, Technische Universität München
    • , Soile TapioAffiliated withInstitute of Radiation Biology, Helmholtz Zentrum München, German Research Center for Environmental Health Email author 

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Abstract

High doses of ionising radiation damage the heart by an as yet unknown mechanism. A concern for radiological protection is the recent epidemiological data indicating that doses as low as 100–500 mGy may induce cardiac damage. The aim of this study was to identify potential molecular targets and/or mechanisms involved in the pathogenesis of low-dose radiation-induced cardiovascular disease. The vascular endothelium plays a pivotal role in the regulation of cardiac function and is therefore a potential target tissue. We report here that low-dose radiation induced rapid and time-dependent changes in the cytoplasmic proteome of the human endothelial cell line EA.hy926. The proteomes were investigated at 4 and 24 h after irradiation at two different dose rates (Co-60 gamma ray total dose 200 mGy; 20 mGy/min and 190 mGy/min) using 2D-DIGE technology. Differentially expressed proteins were identified, after in-gel trypsin digestion, by MALDI-TOF/TOF tandem mass spectrometry, and peptide mass fingerprint analyses. We identified 15 significantly differentially expressed proteins, of which 10 were up-regulated and 5 down-regulated, with more than ± 1.5-fold difference compared with unexposed cells. Pathways influenced by the low-dose exposures included the Ran and RhoA pathways, fatty acid metabolism and stress response.