Radiation and Environmental Biophysics

, Volume 47, Issue 1, pp 157–168

Regulation of Saccharomyces cerevisiae DNA polymerase η transcript and protein

Original Paper

DOI: 10.1007/s00411-007-0132-1

Cite this article as:
Pabla, R., Rozario, D. & Siede, W. Radiat Environ Biophys (2008) 47: 157. doi:10.1007/s00411-007-0132-1

Abstract

RAD30-encoded DNA polymerase η functions as a translesion polymerase that can bypass the most frequent types of UV-induced pyrimidine photoproducts in an error-free manner. Although its transcript is UV-inducible in Saccharomyces cerevisiae, Rad30 (studied as a Rad30-Myc fusion) is a stable protein whose levels do not fluctuate following UV treatment or during cell cycle progression. Rad30 protein is subject to monoubiquitination whose level is upregulated in G1 and downregulated during S-phase reentry. This downregulation is accelerated in UV-treated cells. A missense mutation (L577Q) of the ubiquitin binding domain (UBZ) confers a reduced degree of ubiquitination outside of G1 and a complete failure to stably interact with ubiquitinated substrates. This mutation confers a phenotype resembling a complete RAD30 deletion, thus attesting to the significance of the UBZ motif for polymerase η function in vivo.

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  1. 1.Dept of Cell Biology and GeneticsUniversity of North Texas Health Science Center Fort WorthUSA
  2. 2.Graduate Program in Biomedical SciencesUniversity of North Texas Health Science CenterFort WorthUSA