Lung

, Volume 184, Issue 6, pp 318–323

Preventive Effect of Hochu-ekki-to on Lipopolysaccharide-Induced Acute Lung Injury in BALB/c Mice

  • Shunji Tajima
  • Masashi Bando
  • Hideaki Yamasawa
  • Shoji Ohno
  • Hiroshi Moriyama
  • Toshinori Takada
  • Eiichi Suzuki
  • Fumitake Gejyo
  • Yukihiko Sugiyama
Article

DOI: 10.1007/s00408-006-0018-z

Cite this article as:
Tajima, S., Bando, M., Yamasawa, H. et al. Lung (2006) 184: 318. doi:10.1007/s00408-006-0018-z

Abstract

This study was designed to investigate the effect of Hochu-ekki-to (TJ-41), a Japanese herbal medicine, on the development of lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice. ALI was induced in female BALB/c mice by the intranasal administration of 0.1 mg/kg LPS. The mice were divided into a group receiving normal feed and another group receiving feed mixed with TJ-41 at a dose of 1 g/kg/day for 8 weeks before LPS challenge. In the bronchoalveolar lavage fluid, the preadministration of TJ-41 caused significant reduction in the absolute number of total cells, neutrophils, and macrophages. The preadministration of TJ-41 significantly inhibited increases in the serum level of keratinocyte chemoattractant (KC), which is a murine chemotaxin for neutrophils that corresponds to human interleukin-8, with respect to its concentration at 24 h after LPS challenge. Furthermore, the histopathologic findings indicated that alveolitis with leukocyte infiltration in the alveolar space was less severe in the TJ-41-treated mice than in the control mice. These findings indicated that the preadministration of TJ-41 could show an inhibitory effect on ALI in this experimental murine system associated with the suppression of chemokine production.

Keywords

Acute lung injuryGranulocyte-macrophage colony-stimulating factorHochu-ekki-toKeratinocyte chemoattractantLipopolysaccharide

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Shunji Tajima
    • 1
    • 2
  • Masashi Bando
    • 1
  • Hideaki Yamasawa
    • 1
  • Shoji Ohno
    • 1
  • Hiroshi Moriyama
    • 2
  • Toshinori Takada
    • 2
  • Eiichi Suzuki
    • 2
  • Fumitake Gejyo
    • 2
  • Yukihiko Sugiyama
    • 1
  1. 1.Division of Pulmonary Medicine, Department of MedicineJichi Medical SchoolShimotsukeJapan
  2. 2.Division of Respiratory Medicine, Graduate School of Medical and Dental SciencesNiigata UniversityNiigataJapan