ORIGINAL PAPER

European Archives of Psychiatry and Clinical Neuroscience

, 256:307

First online:

Taenia solium DNA is present in the cerebrospinal fluid of neurocysticercosis patients and can be used for diagnosis

  • C. R. AlmeidaAffiliated withLaboratório de Neurociências (LIM27), Instituto e Departamento de Psiquiatria, Faculdade de Medicina, Universidade de São Paulo (FMUSP)
  • , E. P. OjopiAffiliated withLaboratório de Neurociências (LIM27), Instituto e Departamento de Psiquiatria, Faculdade de Medicina, Universidade de São Paulo (FMUSP)
  • , C. M. NunesAffiliated withDepto de Apoio, Produção e Saúde Animal, UNESP-Araçatuba
  • , L. R. MachadoAffiliated withDepto de Neurologia, FMUSP, Universidade de São Paulo
  • , O. M. TakayanaguiAffiliated withDepto de Neurologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo
  • , J. A. LivramentoAffiliated withDepto de Neurologia, FMUSP, Universidade de São Paulo
  • , R. AbrahamAffiliated withDepto de Medicina, Universidade de Taubaté
  • , W. F. GattazAffiliated withLaboratório de Neurociências (LIM27), Instituto e Departamento de Psiquiatria, Faculdade de Medicina, Universidade de São Paulo (FMUSP)
  • , A. J. VazAffiliated withLaboratório de Imunologia Clínica, Fac. Ciências Farmacêuticas, USP
    • , E. Dias-NetoAffiliated withLaboratório de Neurociências (LIM27), Instituto e Departamento de Psiquiatria, Faculdade de Medicina, Universidade de São Paulo (FMUSP) Email author 

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Abstract

Neurocysticercosis is the most frequent parasitic infection of the CNS and the main cause of acquired epilepsy worldwide. Seizures are the most common symptoms of the disease, together with headache, involuntary movements, psychosis and a global mental deterioration. Absolute diagnostic criteria include the identification of cysticerci, with scolex, in the brain by MRI imaging. We demonstrate here, for the first time, that T. solium DNA is present in the cerebrospinal fluid of patients. The PCR amplification of the parasite DNA in the CSF enabled the correct identification of 29/30 cases (96.7 %). The PCR diagnosis of parasite DNA in the CSF may be a strong support for the diagnosis of neurocysticercosis.

Key words

PCR CSF neurocysticercosis Taenia solium liquor