European Archives of Oto-Rhino-Laryngology

, Volume 270, Issue 8, pp 2231–2237

Protective effect of thymoquinone against cisplatin-induced ototoxicity

  • Mustafa Sagit
  • Ferhat Korkmaz
  • Alper Akcadag
  • Mehmet Akıf Somdas
Otology

DOI: 10.1007/s00405-012-2254-6

Cite this article as:
Sagit, M., Korkmaz, F., Akcadag, A. et al. Eur Arch Otorhinolaryngol (2013) 270: 2231. doi:10.1007/s00405-012-2254-6

Abstract

The aim of this study was to investigate the potential protective effect of thymoquinone against cisplatin-induced ototoxicity. This study is a prospective, controlled experimental animal study. Experiments were performed on 30 healthy female Sprague-Dawley rats. Thirty animals were divided into three groups of 10 animals each. Group 1 received an intraperitoneal (i.p.) injection of cisplatin 15 mg/kg. Group 2 received i.p. thymoquinone 40 mg/kg/day for 2 days prior to cisplatin injection and third day i.p. cisplatin 15 mg/kg was administered concomitantly. Group 2 continued to receive i.p. thymoquinone until fifth day. Group 3 received i.p. thymoquinone 40 mg/kg/day for 5 days. Pretreatment distortion product otoacoustic emissions (DPOAE) and auditory brain stem responses (ABR) testing from both ears were obtained from the animals in all groups. After the baseline measurements, drugs were injected intraperitonally. After an observation period of 3 days, DPOAE measurements and ABR testing were obtained again and compared with the pretreatment values. There was no statistically significant difference between pre and post-treatment DPOAE responses and ABR thresholds group 2 and 3. However, group 1 demonstrated significant deterioration of the ABR thresholds and DPOAE responses. Our results suggest that DPOAE responses and ABR thresholds were preserved in the cisplatin plus TQ-treated group when compared with the group receiving cisplatin alone. According to these results, cisplatin-induced ototoxicity may be prevented by thymoquinone use in rats.

Keywords

Cisplatin Thymoquinone Ototoxicity Distortion product otoacoustic emission Auditory brain stem responses 

Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Mustafa Sagit
    • 1
    • 3
  • Ferhat Korkmaz
    • 1
  • Alper Akcadag
    • 2
  • Mehmet Akıf Somdas
    • 1
  1. 1.Department of ENTKayseri Training and Research HospitalKayseriTurkey
  2. 2.Subdepartment of AudiologyKayseri Training and Research HospitalKayseriTurkey
  3. 3.Kayseri Eğitim ve Araştırma Hastanesi KBB Kliniği, Sanayi Mah.KayseriTurkey

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