European Archives of Oto-Rhino-Laryngology

, Volume 269, Issue 6, pp 1653–1663

Gene expression analysis of SCC tumor cells in muscle tissue

  • Walter Hundt
  • Esther L. Yuh
  • Mykhaylo Burbelko
  • Andreas Kiessling
  • Mark D. Bednarski
  • Silke Steinbach
Article

DOI: 10.1007/s00405-011-1799-0

Cite this article as:
Hundt, W., Yuh, E.L., Burbelko, M. et al. Eur Arch Otorhinolaryngol (2012) 269: 1653. doi:10.1007/s00405-011-1799-0

Abstract

The purpose of this study was to evaluate microarray technology of HNSCC cells in muscle tissue. 200 SCCVII tumor cells were injected intramuscularly into the right flank of ten C3H/Km mice each. One week later the animals were killed and the tissue taken out. Histology (H&E staining) and microarray of the tissue were performed. Histology showed a few tumor cells between the muscle fibers. Microarray technology showed different gene expression pattern of the muscle tissue with SCCVII cells in comparison with normal muscle tissue. Only those genes showing a fold change difference of 5 or higher were considered. Gene expression analysis revealed changes in the expression levels of SCCVII cells in muscle tissue in 220 genes. Significant gene expression differences between SCCVII cells in muscle tissue and pure muscle tissue could be seen.

Keywords

SCCVII tumor cellsMuscle tissueHistologyGene expression

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Walter Hundt
    • 1
  • Esther L. Yuh
    • 2
  • Mykhaylo Burbelko
    • 1
  • Andreas Kiessling
    • 1
  • Mark D. Bednarski
    • 2
  • Silke Steinbach
    • 3
  1. 1.Department of RadiologyPhilipps University MarburgMarburgGermany
  2. 2.Department of Radiology, Lucas CenterStanford UniversityStanfordUSA
  3. 3.Department of Otolaryngology Head and Neck SurgeryPhilipps University MarburgMarburgGermany