A cDNA microarray analysis identifies 52 genes associated with cis-diamminedichloroplatinum susceptibility in head and neck squamous cell carcinoma cell lines

  • Takenori Ogawa
  • Toru Furukawa
  • Kiyoto Shiga
  • Sho Hashimoto
  • Kazumi Ogawa
  • Toshimitsu Kobayashi
  • Akira Horii
Head and Neck

DOI: 10.1007/s00405-009-0976-x

Cite this article as:
Ogawa, T., Furukawa, T., Shiga, K. et al. Eur Arch Otorhinolaryngol (2010) 267: 123. doi:10.1007/s00405-009-0976-x
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Abstract

We analyzed the cis-diamminedichloroplatinum (CDDP) susceptibility of ten head and neck squamous cell carcinoma (HNSCC) cell lines and found that this susceptibility varied significantly among the cell lines. Apoptotic cell death was predominant after the CDDP treatment, and a significant association was observed between the induction of apoptosis and the CDDP susceptibility. An analysis using a cDNA microarray consisting of 23,040 genes identified 52 genes that showed altered expression patterns between super-sensitive and super-resistant cell lines after the CDDP treatments. Using these 52 genes, we successfully distinguished the super-resistant cell lines from others. Our present results give us valuable clues to better understand the chemosensitivities of such cells to CDDP. This will improve the clinical management of patients with HNSCC.

Keywords

CDDP Chemosensitivity HNSCC Microarray 

Supplementary material

405_2009_976_MOESM1_ESM.ppt (68 kb)
The relationships of IC50 values at 24 hours and 1-DT (A), and at 96 hours and 3-DT (B). Results of HSQ89, IMC4, RPMI2650, and IMC3 are shown as typical examples. Note that IC50 values are well correlated in all the cell lines. (PPT 68 kb)
405_2009_976_MOESM2_ESM.ppt (170 kb)
Results of FACS analyses. (A) Cells were treated with CDDP at 10μg/ml and cultured for 1/3, 2/3, 1, or 2 DT. Then the cells were collected, and FACS analyses were performed. SAS and HO-1-u-1 are shown as typical examples of CDDP-sensitive and -resistant cell lines, respectively. (B) Cells were treated with CDDP at indicated concentrations and cultured for 1/3, 2/3, 1, and 2 DT. Then the cells were collected, and FACS analyses were performed. SAS and RPMI2650 are shown at 1 DT, as typical examples of CDDP-sensitive cell lines. Note that sub-G0/G1 fraction decreased at the high concentration of CDDP. (PPT 170 kb)
405_2009_976_MOESM3_ESM.ppt (262 kb)
Expressions of the TP53 and p21 proteins were analyzed by Western blotting after several genotoxic stimuli. A, ADR treatment at 0.6 μg/ml; U, UV at 5mJ/cm3; C3and C10, CDDP treatments at 3 and 10 μg/ml, respectively. ACTB (β-actin) was monitored as the control. (PPT 262 kb)
405_2009_976_MOESM4_ESM.ppt (120 kb)
Results of microarray analyses. (A) Results of cluster analyses at 1/4-DT after 10μg/ml of CDDP treatment. The 2962 selected genes with reliable signalintensities before CDDP treatment were used. Red indicates a quotient of 1 or larger, and blue indicates a quotient of 1 or less. (PPT 119 kb)
405_2009_976_MOESM5_ESM.ppt (551 kb)
Results of microarray analyses. (B) Comparison of the expression levels of 52 genes between SR (left, IMC4 and HO-1-u-1) and SS (right, IMC3 and SAS) cell lines. Duplicatedexperiments were performed, and average value was plotted. (PPT 551 kb)
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Supplementary Table 1 (XLS 25 kb)
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Supplementary Table 2A (XLS 27 kb)
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Supplementary Table 2B (XLS 26 kb)
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Supplementary material Table 2C (XLS 26 kb)
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Supplementary Table 2D (XLS 26 kb)
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Supplementary Table 2E (XLS 25 kb)
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Supplementary Table 2F (XLS 27 kb)

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Takenori Ogawa
    • 1
    • 2
  • Toru Furukawa
    • 1
  • Kiyoto Shiga
    • 2
  • Sho Hashimoto
    • 2
  • Kazumi Ogawa
    • 1
    • 2
  • Toshimitsu Kobayashi
    • 2
  • Akira Horii
    • 1
  1. 1.Department of Molecular PathologyTohoku University School of MedicineSendaiJapan
  2. 2.Department of Otolaryngology and Head and Neck SurgeryTohoku University School of MedicineSendaiJapan

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