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Follicular fluid anti-Müllerian hormone: a predictive marker of fertilization capacity of MII oocytes

  • Gynecologic Endocrinology and Reproductive Medicine
  • Published:
Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Purpose

The present study aimed to correlate anti-Müllerian hormone (AMH) levels in follicular fluid (FF) with oocyte maturity stages, morphological quality of metaphase II (MII) oocyte and fertilization capacity of MII oocytes.

Methods

A total of 92 infertile women undergoing controlled ovarian stimulation and intracytoplasmic sperm injection were analyzed. Patients were divided into two groups according to age: <35 years (n = 43) and ≥35 years (n = 49). An FF sample was obtained from a single dominant follicle in each patient for a total of 92 follicular fluid samples analyzed. AMH levels in serum and follicular fluid were measured by enzyme-linked immunosorbent assay. Mature MII oocytes, zygotes, and embryos were assessed for morphological quality.

Results

Serum AMH levels were significantly higher in patients aged <35 years. No correlation was observed between FF AMH level and oocyte maturation stages or morphological quality of MII oocyte. Significantly lower FF AMH levels were observed in fertilized MII oocytes than in non-fertilized MII oocytes in patients aged <35 years (2.56 ± 2.0 ng/ml vs. 4.81 ± 4.14 ng/ml; p = 0.032).

Conclusions

The present study revealed no correlation between FF AMH and oocyte maturity stage or morphological quality of MII oocyte. However, FF AMH might be a predictive marker for fertilization capacity of MII oocytes.

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Acknowledgments

The authors thank the Department of Histology and Embryology, Rijeka School of Medicine, University of Rijeka for their support during the study. The authors also thank Marina Merkaš for assistance in statistical analysis. This research was funded by the Ministry of Science, Education and Sport of the Republic of Croatia, grant number 62-00000-3553. The funders had no role in study design, collection, analysis, and interpretation of data, writing of the report and decision to submit the article for publication. The authors had no financial relationship with the organization that sponsored the research.

Conflict of interest

The authors declare that they have no conflict of interest. The authors have full control of all primary data and agree to alow the journal to review data if requested.

Ethical standards

The study design was approved by the Ethics Committee of the Clinical Hospital Center Rijeka, and written consent was obtained from all participants prior to their inclusion in the study. The study was performed in accordance with the ethical standards laid down in the 1964 Declaration of Helsinki. The authors have full control of all primary data and agree to alow the journal to review data if requested.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Clinical Hospital Center Rijeka, University of Rijeka, Cambierieva 17/5, 51 000, Rijeka, Croatia

    Tamara Tramišak Milaković, Linda Panić Horvat, Kristina Čavlović, Neda Smiljan Severinski, Hrvoje Vlašić & Ivan Vlastelić

  2. Department of Obstetrics and Gynecology, Clinical Hospital Center “Sestre milosrdnice”, University of Zagreb, Vinogradska cesta 29, 10 000, Zagreb, Croatia

    Dejan Ljiljak

  3. Department of Biology and Medical Genetics, Rijeka School of Medicine, University of Rijeka, Ulica Braće Branchetta 20, 51 000, Rijeka, Croatia

    Anđelka Radojčić Badovinac

  4. Department of Biotechnology, University of Rijeka, Ulica Radmile Matejčić 2, 51 000, Rijeka, Croatia

    Anđelka Radojčić Badovinac

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  1. Tamara Tramišak Milaković
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  2. Linda Panić Horvat
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Correspondence to Tamara Tramišak Milaković.

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Tramišak Milaković, T., Panić Horvat, L., Čavlović, K. et al. Follicular fluid anti-Müllerian hormone: a predictive marker of fertilization capacity of MII oocytes. Arch Gynecol Obstet 291, 681–687 (2015). https://doi.org/10.1007/s00404-014-3460-9

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  • DOI: https://doi.org/10.1007/s00404-014-3460-9

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