Archives of Dermatological Research

, Volume 289, Issue 5, pp 285–291

Binding and in vitro modulation of human epidermal Langerhans cell functions by substance P

  • Véronique Staniek
  • L. Misery
  • Josette Péguet-Navarro
  • Jacques Abello
  • Jean-Daniel Doutremepuich
  • Alain Claudy
  • Daniel Schmitt
Original paper

DOI: 10.1007/s004030050194

Cite this article as:
Staniek, V., Misery, L., Péguet-Navarro, J. et al. Arch Dermatol Res (1997) 289: 285. doi:10.1007/s004030050194

Abstract

Substance P (SP) is distributed in both the central and peripheral nervous system. It has various effects on immunocompetent cells, such as macrophages and lymphocytes. The aim of our study was to search for the presence of SP receptors (SP-R) on human cutaneous Langerhans cells (LC), and to determine the effects of SP on LC immunological functions in a model of mixed epidermal cell-lymphocyte reaction (MELR). Radioligand binding studies showed that LC-enriched epidermal cell suspensions reversibly bound SP, and that the specific binding increased with the percentage of LC. Functional assays showed that SP had no effect when added at concentrations from 10–6M to 10–12M to the MELR. The addition of SP at concentrations of 10–4M and 10–5M was able to inhibit the allogeneic T-cell response (98.3 ± 1.8% and 92.8 ± 8.9% inhibition, respectively) without modifying the cell viability. This inhibition was through an effect of SP on both T-cell and LC function. We conclude that SP has receptors on LC and may inhibit antigen presentation.

Key words Substance PSubstance P receptorsEpidermal Langerhans cellsImmunomodulation

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • Véronique Staniek
    • 1
  • L. Misery
    • 1
  • Josette Péguet-Navarro
    • 1
  • Jacques Abello
    • 2
  • Jean-Daniel Doutremepuich
    • 3
  • Alain Claudy
    • 1
  • Daniel Schmitt
    • 1
  1. 1.INSERM U 346, Pav R Hôpital E. Herriot, F-69437 Lyon Cédex 03, France Fax 72.11.02.90FR
  2. 2.INSERM U 45 Pav Hbis Hôpital E. Herriot, F-69437 Lyon Cédex 03, FranceFR
  3. 3.CIRD-Galderma, 06902 Sophia-Antipolis, FranceFR