Archives of Dermatological Research

, Volume 289, Issue 5, pp 277–284

Interleukin 1β and the stimulation of Langerhans cell migration: comparisons with tumour necrosis factor α

Authors

  • M. Cumberbatch
    • Zeneca Central Toxicology Laboratory, Alderley Park, Macclesfield, Cheshire, SK10 4TJ, UK Tel. 01625 516414; Fax 01625 590249
  • R. J. Dearman
    • Zeneca Central Toxicology Laboratory, Alderley Park, Macclesfield, Cheshire, SK10 4TJ, UK Tel. 01625 516414; Fax 01625 590249
  • I. Kimber
    • Zeneca Central Toxicology Laboratory, Alderley Park, Macclesfield, Cheshire, SK10 4TJ, UK Tel. 01625 516414; Fax 01625 590249
Original paper

DOI: 10.1007/s004030050193

Cite this article as:
Cumberbatch, M., Dearman, R. & Kimber, I. Arch Dermatol Res (1997) 289: 277. doi:10.1007/s004030050193

Abstract

Epidermal Langerhans cells (LC) and the cells into which they mature are believed to play a pivotal role in cutaneous immune function. The induction phase of contact sensitization is associated with the migration of LC from the skin and their accumulation as dendritic cells (DC) in lymph nodes draining the site of exposure. We have demonstrated previously that tumour necrosis factor α (TNF-α), an epidermal cytokine produced by keratinocytes, provides one signal for LC migration. We describe here experiments designed to evaluate the influence of interleukin 1β (IL-1β), a product exclusively of LC in murine epidermis, on LC migration, LC morphology and DC accumulation, and to compare the effects of this cytokine with those of TNF-α. Both cytokines induced a significant reduction in the frequency of epidermal LC and the arrival of DC in draining lymph nodes. Changes in both parameters were induced more rapidly following intradermal administration of TNF-α than were observed after treatment with IL-1β. However, the reduction in LC frequency was more persistent with IL-1β. Both cytokines caused the activation of LC, characterized by the acquisition of a more dendritic morphology and the increased expression of Ia molecules. These results demonstrate that IL-1β and TNF-α can each stimulate the migration of epidermal LC, but that the changes induced by these cytokines are not identical.

Key words Langerhans cellDendritic cellInterleukin 1βTumour necrosis factor αContact sensitization

Copyright information

© Springer-Verlag Berlin Heidelberg 1997