Archives of Dermatological Research

, Volume 292, Issue 6, pp 285–291

Nickel-induced cytokine production from mononuclear cells in nickel-sensitive individuals and controls

Cytokine profiles in nickel-sensitive individuals with nickel allergy-related hand eczema before and after nickel challenge

Authors

  • L. Borg
    • Reference Laboratory, National Institute of Occupational Health, Lersø Parkallé 105, DK-2100 Copenhagen, Denmark e-mail: lbo@ami.dk, Tel.: +45-39-165200, Fax: +45-39-165201
  • Jytte Molin Christensen
    • Reference Laboratory, National Institute of Occupational Health, Lersø Parkallé 105, DK-2100 Copenhagen, Denmark e-mail: lbo@ami.dk, Tel.: +45-39-165200, Fax: +45-39-165201
  • Jesper Kristiansen
    • Reference Laboratory, National Institute of Occupational Health, Lersø Parkallé 105, DK-2100 Copenhagen, Denmark e-mail: lbo@ami.dk, Tel.: +45-39-165200, Fax: +45-39-165201
  • Niels Henrik Nielsen
    • Department of Dermatology, Gentofte Hospital, University of Copenhagen, Niels Andersens Vej 65, DK-2900 Hellerup, Denmark
  • Torkil Menné
    • Department of Dermatology, Gentofte Hospital, University of Copenhagen, Niels Andersens Vej 65, DK-2900 Hellerup, Denmark
  • Lars K. Poulsen
    • National University Hospital, Laboratory of Medical Allergology, Allergy Unit, TA 7542, Tagensvej 20, DK-2200 Copenhagen, Denmark
Original paper

DOI: 10.1007/s004030000129

Cite this article as:
Borg, L., Christensen, J., Kristiansen, J. et al. Arch Dermatol Res (2000) 292: 285. doi:10.1007/s004030000129

Abstract Exposure to nickel is a major cause of allergic contact dermatitis which is considered to be an inflammatory response induced by antigen-specific T cells. Here we describe the in vitro analysis of the nickel-specific T-cell-derived cytokine response of peripheral blood mononuclear cells from 35 nickel-allergic and 30 non-nickel-allergic individuals. Peripheral blood mononuclear cells were stimulated with 10–4 and 10–5 mol/l NiSO4 for 6 days and then additionally with ionomycin and phorbol myristate acetate for 24 h. Culture supernatants were analysed for interleukin-4 (IL-4), IL-5, interferon-γ (IFN-γ) and tumour necrosis factor-α (TNF-α) by quantitative ELISA. The analysis showed that the synthesis of IL-4 and IL-5 but not of IFN-γ or TNF-α was significantly higher in the nickel-allergic individuals. The finding of preferential synthesis of Th2 cytokines was somewhat of a surprise, since previous studies have suggested a Th1 response in nickel-mediated allergic contact dermatitis. Subsequently, the nickel-allergic individuals were randomized to experimental exposure to nickel or vehicle in a double-blind design. A daily 10-min exposure of one finger to 10 ppm nickel solution for 1 week followed by 100 ppm for an additional week evoked a clinical response of hand eczema in the nickel-exposed group. Blood samples were drawn on days 7 and 14 after the start of this exposure to occupationally relevant concentrations of nickel. No statistically significant differences were observed in the nickel-induced in vitro cytokine response during the exposure period. Our results indicate the possibility that IL-4 and IL-5 are involved in the pathogenesis of nickel-mediated contact dermatitis.

Key words NickelAllergic contact dermatitisT cellsIL-4IL-5

Copyright information

© Springer-Verlag Berlin Heidelberg 2000