Archives of Dermatological Research

, 301:381

A cell-based system for screening hair growth-promoting agents

  • Sungran Huh
  • Jongsung Lee
  • Eunsun Jung
  • Sang-Cheol Kim
  • Jung-Il Kang
  • Jienny Lee
  • Yong-Woo Kim
  • Young Kwan Sung
  • Hee-Kyoung Kang
  • Deokhoon Park
Short Communication

DOI: 10.1007/s00403-009-0931-0

Cite this article as:
Huh, S., Lee, J., Jung, E. et al. Arch Dermatol Res (2009) 301: 381. doi:10.1007/s00403-009-0931-0

Abstract

Androgen-inducible transforming growth factor β (TGF-β1) derived from dermal papilla cells (DPCs) is a catagen inducer that mediates hair growth suppression in androgenetic alopecia (AGA). In this study, a cell-based assay system was developed to monitor TGF-β1 promoter activity and then used to evaluate the effects of activated TGF-β1 promoter in human epidermal keratinocytes (HaCaT). To accomplish this, a pMetLuc-TGF-β1 promoter plasmid that expresses the luciferase reporter gene in response to TGF-β1 promoter activity was constructed. Treatment of HaCaT with dihydrotestosterone, which is known to be a primary factor of AGA, resulted in a concentration-dependent increase in TGF-β1 promoter activity. However, treatment of HaCaT with the TGF-β1 inhibitor, curcumin, resulted in a concentration-dependant decrease in TGF-β1 expression. Subsequent use of this assay system to screen TGF-β1 revealed that HaCaT that were treated with apigenin showed decreased levels of TGF-β1 expression. In addition, treatment with apigenin also significantly increased the proliferation of both SV40T-DPCs (human DPCs) and HaCaT cells. Furthermore, apigenin stimulated the elongation of hair follicles in a rat vibrissa hair follicle organ culture. Taken together, these findings suggest that apigenin, which is known to have antioxidant, anti-inflammatory, and anti-tumor properties, stimulates hair growth through downregulation of the TGF-β1 gene. In addition, these results suggest that this assay system could be used to quantitatively measure TGF-β1 promoter activity in HaCaT, thereby facilitating the screening of agents promoting hair growth.

Keywords

TGF-β1Hair growthApigeninDermal papilla cellHair follicle

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Sungran Huh
    • 1
  • Jongsung Lee
    • 1
  • Eunsun Jung
    • 1
  • Sang-Cheol Kim
    • 2
  • Jung-Il Kang
    • 2
  • Jienny Lee
    • 1
  • Yong-Woo Kim
    • 1
  • Young Kwan Sung
    • 3
  • Hee-Kyoung Kang
    • 2
  • Deokhoon Park
    • 1
  1. 1.Biospectrum Life Science InstituteGyeonggi-doRepublic of Korea
  2. 2.Department of PharmacologyCheju National University School of MedicineJeju CityRepublic of Korea
  3. 3.Department of Immunology, School of MedicineKyungpook National UniversityDaeguRepublic of Korea