Archives of Dermatological Research

, Volume 301, Issue 2, pp 139–149

Angiogenin is expressed in human dermal papilla cells and stimulates hair growth

Original Paper

DOI: 10.1007/s00403-008-0907-5

Cite this article as:
Zhou, N., Fan, W. & Li, M. Arch Dermatol Res (2009) 301: 139. doi:10.1007/s00403-008-0907-5


The perifollicular vasculature undergoes hair-cycle dependent expansion and degeneration. Multiple soluble factors derived from dermal papilla cells (DPCs) may act on surrounding blood vessels to influence angiogenesis, growth and differentiation, and thereby regulate cyclic hair growth. The goal of this study was to examine the expression of angiogenin, a potent angiogenic factor, in human DPCs, and to determine its role in hair growth. Reverse transcription polymerase chain reaction (RT-PCR), western blotting, immunofluorescence and ELISA analyzes were used to investigate the expression of angiogenin in human DPCs, while semi-quantitative RT-PCR was used to assess angiogenin mRNA expression in murine skin phased at different stages of the hair cycle. We detected angiogenin expression in DPCs, where it was found to be localized to the cytoplasm. Angiogenin mRNA was expressed in murine skin in a hair-cycle dependent manner, with maximum levels observed at the late anagen. Local injection of angiogenin promoted skin angiogenesis and induced anagen VI. In vitro studies showed that angiogenin significantly enhanced the elongation of hair follicles, and stimulated DPCs and ORS keratinocytes to proliferate. Taken together, these findings show that angiogenin is expressed in human DPCs, where it might contribute to hair growth directly, by stimulating DPCs and ORS keratinocytes to proliferate, or indirectly, by inducing local vascularization.


Angiogenesis Angiogenin Dermal papilla cell Hair cycle Hair follicle 



Dermal papilla cell


Connective tissue sheath


Human skin fibroblast


Outer root sheath


Fetal calf serum


Vascular endothelial growth factor


Bovine serum albumin


Phosphate buffered saline

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  1. 1.Department of DermatologyThe First Affiliated Hospital of Nanjing Medical UniversityNanjingChina

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