Archives of Dermatological Research

, Volume 299, Issue 1, pp 13–24

Transduction of β3 integrin subunit cDNA confers on human keratinocytes the ability to adhere to gelatin

  • Miyoko Kubo
  • Richard A. F. Clark
  • Anne B. Katz
  • Lorne B. Taichman
  • Zaishun Jin
  • Ying Zhao
  • Takahiko Moriguchi
Original Paper

DOI: 10.1007/s00403-006-0718-5

Cite this article as:
Kubo, M., Clark, R.A.F., Katz, A.B. et al. Arch Dermatol Res (2007) 299: 13. doi:10.1007/s00403-006-0718-5
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Abstract

αvβ3 is a multiligand integrin receptor that interacts with fibrinogen (FG), fibrin (FB), fibronectin (FN), vitronectin (VN), and denatured collagen. We previously reported that cultured normal human keratinocytes, like in vivo keratinocytes, do not express αvβ3 on the cell surface, and do not adhere to and migrate on FG and FB. Furthermore, we reported that human keratinocytes transduced with β3 integrin subunit cDNA by a retrovirus-mediated transduction method express αvβ3 on the cell surface and adhere to FG, FB, FN, and VN significantly compared with β-galactosidase (β-gal) cDNA-transduced keratinocytes (control). In this study, we determined whether these β3 integrin subunit cDNA-transduced keratinocytes or normal human keratinocytes adhere to denatured collagen (gelatin) using a 1 h cell adhesion assay. β3 cDNA-transduced keratinocytes adhered to gelatin, whereas no significant adhesion was observed with the control cells (β-gal cDNA-transduced keratinocytes and normal human keratinocytes). The adhesion to gelatin was inhibited by LM609, a monoclonal antibody to αvβ3, and RGD peptides but not by normal mouse IgG1 nor RGE peptides. Thus, transduction of β3 integrin subunit cDNA confers on human keratinocytes the ability to adhere to denatured collagen (gelatin) as well as to FG, FB, VN, and FN. Otherwise, normal human keratinocytes do not adhere to gelatin. These data support the idea that β3 cDNA-transduced human keratinocytes can be a good material for cultured epithelium to achieve better take rate with acute or chronic wounds, in which FG, FB, and denatured collagen are abundantly present.

Keywords

Keratinocytes αvβ3 Transduction Gelatin Cultured epithelium 

Abbreviations

KBM

Keratinocyte basal medium

KGM

Keratinocyte growth medium

PS

Penicillin and streptomycin

β-gal

β-Galactosidase

GEL

Gelatin

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Miyoko Kubo
    • 1
  • Richard A. F. Clark
    • 2
  • Anne B. Katz
    • 3
  • Lorne B. Taichman
    • 3
  • Zaishun Jin
    • 4
  • Ying Zhao
    • 1
  • Takahiko Moriguchi
    • 1
  1. 1.Department of Plastic and Reconstructive SurgeryKawasaki Medical SchoolKurashiki CityJapan
  2. 2.Director of Center for Tissue Engineering, Department of Biomedical Engineering, Dermatology and Medicine, School of MedicineSUNY at Stony BrookStony BrookUSA
  3. 3.Department of Oral Biology and Pathology, School of Dental MedicineSUNY at Stony BrookStony BrookUSA
  4. 4.Deparment of PathologyMudanjiang Medical UniversityMudanjiang CityChina

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