Archives of Dermatological Research

, 298:175

Mixture of sugar and povidone--iodine stimulates wound healing by activating keratinocytes and fibroblast functions

Authors

  • Hiroshi Nakao
    • Pharmacology Group, Fuji Research Laboratories, Pharmaceutical DivisionKowa Company Ltd
  • Masashi Yamazaki
    • Department of DermatologyTokyo Medical University
    • Department of DermatologyTokyo Medical University
  • Hideoki Ogawa
    • Department of DermatologyJuntendo University School of Medicine
Original Paper

DOI: 10.1007/s00403-006-0683-z

Cite this article as:
Nakao, H., Yamazaki, M., Tsuboi, R. et al. Arch Dermatol Res (2006) 298: 175. doi:10.1007/s00403-006-0683-z

Abstract

The topical application of a mixture of sugar and povidone--iodine (PI) has been reported to accelerate the healing of cutaneous wounds and ulcers by promoting re-epithelialization and granulation tissue formation as well as having an anti-microbial effect. To clarify the mechanisms accounting for the efficacy of a 70% sugar and 3% PI paste (U-PASTA™) (SP), various keratinocytes and fibroblasts functions, including proliferation, collagen synthesis, integrin expression, and cytokine and proteinase secretions in the presence of SP were investigated. Cultured human keratinocytes and fibroblasts were treated with various concentrations of SP, SU and PI. The secretion of urokinase-type plasminogen activator (u-PA), transforming growth factor (TGF)-α and interleukin-1α from keratinocytes, was detected by ELISA. Collagen synthesis of fibroblasts was examined by means of detecting proline uptake. Furthermore, integrin expressions of these cells were analyzed using a flow cytometer. SP and PI increased intra-cellular u-PA of keratinocytes and stimulated the secretion of u-PA and TGF-α. Sugar accelerated the extra-cellular u-PA level only. Both SP and sugar increased the collagen synthesis of fibroblasts. SP and PI also remarkably induced the expressions of extra-cellular matrix receptor integrins, α1, α2, α3, α4, α5 and β1, on the surface of keratinocytes and fibroblasts. SP, the mixture of sugar and PI, is likely to act on wounds not only as an antibiotic agent, but also as a modulator for keratinocytes and fibroblasts.

Keywords

Topical agent Cytokine Integrin Collagen Plasminogen activator

Abbreviations

IL-1α

Interleukin-1α

PI

Povidone--iodine;

SU

A paste comprising 70% sugar

SP

A paste comprising 70% sugar and 3% PI

TGF-α

Transforming growth factor-α

TGF-β

Transforming growth factor-β

u-PA

Urokinase-type plasminogen activator

Copyright information

© Springer-Verlag 2006