Original Paper

Archives of Dermatological Research

, 298:175

First online:

Mixture of sugar and povidone--iodine stimulates wound healing by activating keratinocytes and fibroblast functions

  • Hiroshi NakaoAffiliated withPharmacology Group, Fuji Research Laboratories, Pharmaceutical Division, Kowa Company Ltd
  • , Masashi YamazakiAffiliated withDepartment of Dermatology, Tokyo Medical University
  • , Ryoji TsuboiAffiliated withDepartment of Dermatology, Tokyo Medical University Email author 
  • , Hideoki OgawaAffiliated withDepartment of Dermatology, Juntendo University School of Medicine

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access


The topical application of a mixture of sugar and povidone--iodine (PI) has been reported to accelerate the healing of cutaneous wounds and ulcers by promoting re-epithelialization and granulation tissue formation as well as having an anti-microbial effect. To clarify the mechanisms accounting for the efficacy of a 70% sugar and 3% PI paste (U-PASTA™) (SP), various keratinocytes and fibroblasts functions, including proliferation, collagen synthesis, integrin expression, and cytokine and proteinase secretions in the presence of SP were investigated. Cultured human keratinocytes and fibroblasts were treated with various concentrations of SP, SU and PI. The secretion of urokinase-type plasminogen activator (u-PA), transforming growth factor (TGF)-α and interleukin-1α from keratinocytes, was detected by ELISA. Collagen synthesis of fibroblasts was examined by means of detecting proline uptake. Furthermore, integrin expressions of these cells were analyzed using a flow cytometer. SP and PI increased intra-cellular u-PA of keratinocytes and stimulated the secretion of u-PA and TGF-α. Sugar accelerated the extra-cellular u-PA level only. Both SP and sugar increased the collagen synthesis of fibroblasts. SP and PI also remarkably induced the expressions of extra-cellular matrix receptor integrins, α1, α2, α3, α4, α5 and β1, on the surface of keratinocytes and fibroblasts. SP, the mixture of sugar and PI, is likely to act on wounds not only as an antibiotic agent, but also as a modulator for keratinocytes and fibroblasts.


Topical agent Cytokine Integrin Collagen Plasminogen activator