Archives of Dermatological Research

, Volume 297, Issue 6, pp 249–255

The changes in expression of ICAM-3, Ki-67, PCNA, and CD31 in psoriatic lesions before and after methotrexate treatment

  • Ayca Cordan Yazici
  • Umit Tursen
  • Duygu Dusmez Apa
  • Guliz Ikizoglu
  • Hale Api
  • Kiymet Baz
  • Bahar Tasdelen
Original Paper

DOI: 10.1007/s00403-005-0602-8

Cite this article as:
Yazici, A.C., Tursen, U., Apa, D.D. et al. Arch Dermatol Res (2005) 297: 249. doi:10.1007/s00403-005-0602-8

Abstract

Although the effectiveness of methotrexate (MTX) in the treatment of psoriasis is very well established, the mechanism of action is poorly understood. It was suggested that the therapeutic effect of MTX in psoriasis might be mediated by inhibition of adhesion molecule expression. The aim of our study was to investigate the different effects of MTX treatment on cell proliferation, inflammatory infiltrate, adhesion molecules, and angiogenesis in psoriasis, and to clarify the mechanism by which MTX exerts its therapeutic effects. Clinical response, the morpho–phenotypic changes, epidermal thickness, and mitosis count were analyzed and the expression of CD31 and ICAM-3, proliferative markers such as Ki-67, PCNA, were evaluated by immunohistochemical techniques in lesional psoriatic epidermis, before and after the treatment with MTX in ten patients. In posttreatment biopsies a decrease in the degree of epidermal hyperplasia and a significant reduction in the severity of the inflammatory infiltrate (P<0.05) were observed. In addition, CD31 and ICAM-3 expression was significantly decreased on dermal cellular infiltrate, (respectively; P<0.05, P<0.01). Ki67 and PCNA expression were suppressed concurrently in about 90% of cases (P<0.01). We suggest that MTX may have an inhibitory effect on an initial integral component of the pathways that lead to psoriasis. Immunopharmacologic intervention in adhesion event has the potential to improve psoriasis. Inhibition of revascularization may be another mechanism of action of MTX.

Keywords

PsoriasisICAM-3CD31Ki-67PCNAMethotrexate

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Ayca Cordan Yazici
    • 1
  • Umit Tursen
    • 1
  • Duygu Dusmez Apa
    • 2
  • Guliz Ikizoglu
    • 1
  • Hale Api
    • 1
  • Kiymet Baz
    • 1
  • Bahar Tasdelen
    • 3
  1. 1.Departments Of DermatologyMersin University, School of MedicineMersinTurkey
  2. 2.Departments Of PathologyMersin University, School of MedicineMersinTurkey
  3. 3.Departments Of BiostatisticsMersin University, School of MedicineMersinTurkey