Original Paper

Archives of Dermatological Research

, Volume 296, Issue 3, pp 120-124

First online:

Differentiation-regulated expression of Toll-like receptors 2 and 4 in HaCaT keratinocytes

  • Andor PivarcsiAffiliated withDepartment of Dermatology and Allergology, University of SzegedDermatological Research Group of the Hungarian Academy of Sciences Email author 
  • , Andrea KoreckAffiliated withDepartment of Dermatology and Allergology, University of Szeged
  • , Laszlo BodaiAffiliated withDepartment of Dermatology and Allergology, University of Szeged
  • , Márta SzéllAffiliated withDermatological Research Group of the Hungarian Academy of Sciences
  • , Csilla SzegAffiliated withDepartment of Dermatology and Allergology, University of Szeged
  • , Nóra BelsőAffiliated withDepartment of Dermatology and Allergology, University of Szeged
  • , Anna Kenderessy-SzabóAffiliated withDepartment of Dermatology and Allergology, University of Szeged
  • , Zsuzsanna Bata-CsörgőAffiliated withDepartment of Dermatology and Allergology, University of SzegedDermatological Research Group of the Hungarian Academy of Sciences
  • , Attila DobozyAffiliated withDepartment of Dermatology and Allergology, University of SzegedDermatological Research Group of the Hungarian Academy of Sciences
    • , Lajos KeményAffiliated withDepartment of Dermatology and Allergology, University of SzegedDermatological Research Group of the Hungarian Academy of Sciences

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Abstract

Toll-like receptors (TLRs) play an important role in the recognition of pathogens in keratinocytes. In this study, we investigated whether the differentiation state of HaCaT keratinocytes correlates with the expression of TLR2 and TLR4 genes. The expression levels of TLR2 and TLR4 in a HaCaT differentiation model system were determined using quantitative real-time RT-PCR (Q-RT-PCR) and flow cytometry. The progression of keratinocyte differentiation was monitored by determining the level of involucrin gene expression using Q-RT-PCR. The expression levels of TLR2 and TLR4 increased with the stage of differentiation and there were strong correlations between the expression level of the involucrin gene and those of the TLR2 gene (r=0.809, P<0.0001) and the TLR4 gene (r=0.568, P<0.02). Increased cell surface expression of TLR2 and TLR4 was also found in differentiated HaCaT keratinocytes by flow cytometric analysis. Our findings suggest that upregulation of TLR expression during differentiation in keratinocytes could be a part of the differentiation process of keratinocytes and could have biological significance in protecting skin against microbes.

Keywords

Toll-like receptor Epidermis Host defense Innate immunity Keratinocyte