Archives of Orthopaedic and Trauma Surgery

, Volume 120, Issue 5, pp 328–332

Increased interleukin-8 (IL-8) expression is related to aseptic loosening of total hip replacement

Authors

  • J. Lassus
    • Department of Orthopaedics and Traumatology, Helsinki University Central Hospital, Topeliuksenkatu 5, FIN-00260 Helsinki, Finland e-mail: jan.lassus@soy-foa.org, Tel.: +35-894711, Fax: +35-8941248300
  • Ville Waris
    • Institute of Biomedicine, Department of Anatomy, University of Helsinki, Helsinki, Finland
  • Jing-Wen Xu
    • Department of Orthopaedics and Traumatology, Helsinki University Central Hospital, Topeliuksenkatu 5, FIN-00260 Helsinki, Finland e-mail: jan.lassus@soy-foa.org, Tel.: +35-894711, Fax: +35-8941248300
  • Tian-Fang Li
    • Department of Orthopaedics and Traumatology, Helsinki University Central Hospital, Topeliuksenkatu 5, FIN-00260 Helsinki, Finland e-mail: jan.lassus@soy-foa.org, Tel.: +35-894711, Fax: +35-8941248300
  • Jumang Hao
    • National Agency for Medicine, Medical Devices Centre, Helsinki, Finland
  • Yrjänä Nietosvaara
    • Department of Orthopaedics and Traumatology, Helsinki University Central Hospital, Topeliuksenkatu 5, FIN-00260 Helsinki, Finland e-mail: jan.lassus@soy-foa.org, Tel.: +35-894711, Fax: +35-8941248300
  • Seppo Santavirta
    • Department of Orthopaedics and Traumatology, Helsinki University Central Hospital, Topeliuksenkatu 5, FIN-00260 Helsinki, Finland e-mail: jan.lassus@soy-foa.org, Tel.: +35-894711, Fax: +35-8941248300
  • Yrjö T. Konttinen
    • Finnish Academy, Institute of Biomedicine, Department of Anatomy, University of Helsinki and Division of Rheumatology, Department of Medicine, Helsinki University Central Hospital, Helsinki, Finland
Clinical and experimental forum

DOI: 10.1007/s004020050475

Cite this article as:
Lassus, J., Waris, V., Xu, J. et al. Arch Orth Traum Surg (2000) 120: 328. doi:10.1007/s004020050475

Abstract

Aseptic loosening is an increasing problem in total hip replacement (THR). Chronic inflammatory reaction against implant wear particle results in collageno- and osteolysis, leading to loosening of the implant. Cytokines are known to play a major role in this particular inflammatory process [10]. The aim of the present study was to examine interleukin-8 (IL-8) in the synovial-like interface membrane (SLIM) and pseudocapsular tissue of THRs and to compare it to normal knee synovial membrane. Eleven patients suffering from aseptically loosened THRs were included. All the SLIM and pseudocapsular tissue samples were obtained during revision operations. Ten control samples of normal synovium were collected per arthroscopy from the superior recessus of the knee. For immunohistochemical IL-8 detection, polyclonal mouse anti-human immunoglobulin (Ig)G1 IL-8-primary antibody was used with the alkaline phosphatase anti-alkaline phosphatase (APAAP) method. Results were quantitated using the Vidas image analysis system. The highest count levels (mean ± SEM) were detected in SLIM tissue (386 ± 82 cells/mm2). The difference was statistically significant compared with pseudocapsular tissue (193 ± 36 cells/mm2) and control samples (18 ± 5 cells/mm2). Count levels in control tissue were on average 5% of the SLIM tissues values. The present study determines for the first time the cellular origin of IL-8 in aseptically loosened THRs and also quantitates the IL-8-producing cells in the periprosthetic tissue. The results reveal a high rise in IL-8 concentration in SLIM and in synovial tissues. This finding moves us one step forward in solving the complex network of multiple factors affecting loosening of hip implants.

Key words Interleukin-8Aseptic looseningTotal hip replacementImmunohistochemistry

Copyright information

© Springer-Verlag Berlin Heidelberg 2000